Characterization of mango juice by high-resolution NMR, hyphenated NMR, and diffusion-ordered spectroscopy
authors Duarte, IF; Goodfellow, BJ; Gil, AM; Delgadillo, I
nationality International
journal SPECTROSCOPY LETTERS
author keywords DOSY; juice; LC-NMR; NMR; ripening
keywords NUCLEAR-MAGNETIC-RESONANCE; HAIRY RAMIFIED REGIONS; LIQUID FOODS; IDENTIFICATION; RHAMNOGALACTURONASE; SPECTROMETRY; PECTINS; ACIDS; BEER; H-1
abstract The application of nuclear magnetic resonance (NMR) spectroscopy, hyphenated NMR, and diffusion-ordered spectroscopy (DOSY) to the characterization of mango juice, as an example of a complex food mixture, is described. The compositional changes taking place as a function of ripening were followed, and selected metabolites were quantified by integration of the corresponding NMR peaks. In this way, an overall view of the metabolite changes is obtained, enabling the study of the biochemical mechanisms involved in the ripening process. More than 50 compounds were identified by 1D- and 2D-NMR, but many ambiguous assignments remain due to spectral overlap or insufficient coupling information. The use of Liquid Chromatography (LC-NMR) and LC-NMR/Mass Spectrometry (MS) enables a fuller characterization of the soluble pectin fraction to be made; its dependence on ripening stage is discussed. Finally, DOSY adds information on the M(r) of many metabolites, including the pectin fractions of ripe and unripe mango juices, and enables further peak assignments to be made.
publisher TAYLOR & FRANCIS INC
issn 0038-7010
year published 2005
volume 38
issue 3
beginning page 319
ending page 342
digital object identifier (doi) 10.1081/SL-200058713
web of science category Spectroscopy
subject category Spectroscopy
unique article identifier WOS:000229612000009
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journal analysis (jcr 2017):
journal impact factor 0.896
5 year journal impact factor 0.843
category normalized journal impact factor percentile 24.419
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