Optimization of oxygen mass transfer in a multiphase bioreactor with perfluorodecalin as a second liquid phase
authors Amaral, PFF; Freire, MG; Rocha-Leao, MHM; Marrucho, IM; Coutinho, JAP; Coelho, MAZ
nationality International
journal BIOTECHNOLOGY AND BIOENGINEERING
author keywords multiphase bioreactors; aeration; oxygen mass transfer coefficient; Yarrowia lipolytica; perfluorodecalin; olive oil
keywords STIRRED BIOREACTORS; LIPASE PRODUCTION; PERFLUOROCARBON EMULSIONS; ANTIBIOTIC PRODUCTION; TRANSFER COEFFICIENT; SYSTEMS; ENHANCEMENT; SOLUBILITY; BROTHS; LIPOLYTICA
abstract Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (k(L)a). The operational conditions of a stirred, submerged aerated 2-L bioreactor have been optimized by studying the influence of a second liquid phase with higher oxygen affinity (perfluorodecalin or olive oil) in the kLa. Using kLa measurements, the influence of the following parameters on the oxygen transfer rate was evalualed: the volume of working medium, the type of impellers and their position, the organic phase concentration, the aqueous phase composition, and the concentration of inactive biomass. This study shows that the best experimental conditions were achieved with a perfluorodecalin volume fraction of 0.20, mixing using two Rushton turbines with six vertical blades and in the presence of YPD medium as the aqueous phase, with a kLa value of 64.6 h(-1). The addition of 20% of perfluorodecalin in these conditions provided a kLa enhancement of 25% when pure water was the aqueous phase and a 230% enhancement when YPD medium was used in comparison to their respective controls (no perfluorodecalin). Furthermore it is shown that the presence of olive oil as a second liquid phase is not beneficial to the oxygen transfer rate enhancement, leading to a decrease in the kLa values for all the concentrations studied. It was also observed that the magnitude of the enhancement of the kLa values by perfluorodecalin depends on the biomass concentration present.
publisher JOHN WILEY & SONS INC
issn 0006-3592
year published 2008
volume 99
issue 3
beginning page 588
ending page 598
digital object identifier (doi) 10.1002/bit.21640
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000252511800012
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journal analysis (jcr 2017):
journal impact factor 3.952
5 year journal impact factor 4.206
category normalized journal impact factor percentile 80.435
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