H-1 NMR Based Metabonomics of Human Amniotic Fluid for the Metabolic Characterization of Fetus Malformations
authors Graca, G; Duarte, IF; Barros, AS; Goodfellow, BJ; Diaz, S; Carreira, IM; Couceiro, AB; Galhano, E; Gil, AM
nationality International
journal JOURNAL OF PROTEOME RESEARCH
author keywords amniotic fluid; fetus malformations; NMR spectroscopy; chemometrics; metabonomics
keywords MAGNETIC-RESONANCE-SPECTROSCOPY; RESEARCH-AND-DEVELOPMENT; NMR-SPECTROSCOPY; BLOOD-PLASMA; FETAL; URINE; PREGNANCY; PROBE; 2ND; MS
abstract An NMR-metabonomic study of malformed fetuses was carried out through human amniotic fluid (HAF) analysis. Over 70 compounds were detected in control HAF by NMR. Possible confounding variables (fetus gender and gestational and maternal ages) were shown not to induce detectable compositional trends in the control group considered, Malformed fetuses showed variations in glucose, some amino acids and organic acids and proteins. In tandem with enzymatic assays, these NMR results suggest that changes in gycolysis and gluconeogenesis as well as kidney underdevelopment occur in the malformed fetuses studied here.
publisher AMER CHEMICAL SOC
issn 1535-3893
year published 2009
volume 8
issue 8
beginning page 4144
ending page 4150
digital object identifier (doi) 10.1021/pr900386f
web of science category Biochemical Research Methods
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000268661800037

Apoio

1suponsers_list_ciceco.jpg