Analysis of Non-Aromatic Organic Acids in Beer by CE and Direct Detection Mode with Diode Array Detection
authors Erny, GL; Rodrigues, JEA; Gil, AM; Barros, AS; Esteves, VI
nationality International
journal CHROMATOGRAPHIA
author keywords Capillary electrophoresis; Diode array detection; Organic acids in beer; Polybrene
keywords CAPILLARY-ZONE-ELECTROPHORESIS; ANIONS; WINE
abstract A method for the analysis of the main non-aromatic organic acids in beer using capillary electrophoresis is presented. In this work, malic, citric, succinic, pyruvic, acetic and lactic acids are separated using a sodium hydrogen phosphate background electrolyte with direct detection mode with a diode array detector. The separation exhibits lower sensitivity than equivalent methods with indirect detection mode, however, the risk of co-migration with unknown compounds in beer matrixes is significantly reduced. This is due to (i) a higher efficiency (250,000-400,000 theoretical plates), (ii) a higher selectivity than any equivalent method using an indirect detection mode, and (iii) the possibility to monitor other wavelengths in parallel (260 nm for example) to check for possible co-migration with phenolic or benzoic acids. This was critical when working with beer samples as an unknown compound absorbing at 200 and 260 nm was detected in the neighbourhood of malic, citric and succinic acids. Such co-migration will not have been detected using single wavelength detection below 200 nm or indirect detection mode.
publisher VIEWEG
issn 0009-5893
year published 2009
volume 70
issue 11-12
beginning page 1737
ending page 1742
digital object identifier (doi) 10.1365/s10337-009-1377-4
web of science category Biochemical Research Methods; Chemistry, Analytical
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000272173800033
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journal impact factor 1.401
5 year journal impact factor 1.391
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