BIOLOGICAL RESPONSES OF BRUSHITE-FORMING Zn- AND ZnSr-SUBSTITUTED beta-TRICALCIUM PHOSPHATE BONE CEMENTS
authors Pina, S; Vieira, SI; Rego, P; Torres, PMC; Silva, OABDE; Silva, EFDE; Ferreira, JMF
nationality International
journal EUROPEAN CELLS & MATERIALS
author keywords Zinc; strontium; brushite cement; osteoblast proliferation; alkaline phosphatase; MC3T3-E1 cell adhesion; Type-1 collagen; trabecular bone regeneration
keywords CALCIUM-PHOSPHATE; IN-VIVO; ZINC RELEASE; STRONTIUM; DIFFERENTIATION; INJECTABILITY; CYTOCOMPATIBILITY; HYDROXYAPATITE; PROLIFERATION; BIOMATERIAL
abstract The core aim of this study was to investigate zinc (Zn)and zinc and strontium (ZnSr)-containing brushite-forming beta-tricalcium phosphate (TCP) cements for their effects on proliferation and differentiation of osteoblastic-like cells (MC3T3-E1 cell line) as well as for their in vivo behaviour in trabecular bone cylindrical defects in a pilot study. In vitro proliferation and maturation responses of MC3T3-El osteoblastic-like cells to bone cements were studied at the cellular and molecular levels. The Zn- and Sr-containing brushite cements were found to stimulate pre-osteoblastic proliferation and osteoblastic maturation. Indeed, MC3T3-E1 cells exposed to the powdered cements had increased proliferative rates and higher adhesiveness capacity, in comparison to control cells. Furthermore, they exhibited higher alkaline phosphatase (ALP) activity and increased Type-I collagen secretion and fibre deposition into the extracellular matrix. Proliferative and collagen deposition properties were more evident for cells grown in cements doped with Sr. The in vivo osteoconductivc properties of the ZnCPC and ZnSrCPC cements were also pursued. Histological and histomorphometric analyses were performed at 1 and 2 months after implantation, using carbonated apatite cement (Norian SRS (R)) as control. There was no evidence of cement-induced adverse foreign body reactions, and furthermore ZnCPC and ZnSrCPC cements revealed better in vivo performance in comparison to the control apatite cement. Additionally, the presence of both zinc and strontium resulted in the highest I:ate of new bone formation, These novel results indicate that the investigated ZnCPC and ZnSrCPC cements are both biocompatible and osteoconductive, being good candidate materials to use as bone substitutes.
publisher AO RESEARCH INSTITUTE DAVOS-ARI
issn 1473-2262
year published 2010
volume 20
beginning page 162
ending page 177
web of science category Cell & Tissue Engineering; Engineering, Biomedical; Materials Science, Biomaterials
subject category Cell Biology; Engineering; Materials Science
unique article identifier WOS:000282006700011
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journal analysis (jcr 2017):
journal impact factor 3.667
5 year journal impact factor 4.842
category normalized journal impact factor percentile 68.031
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