|
authors |
Ferreira, JA; Azevedo, NF; Vieira, MJ; Figueiredo, C; Goodfellow, BJ; Monteiro, MA; Coimbra, MA |
|
nationality |
International |
|
journal |
CARBOHYDRATE RESEARCH |
|
author keywords |
Cell-surface glycans; Helicobacter pylori; Lipopolysaccharide; Mannan; Vaccine |
|
keywords |
MANNOSE-BINDING LECTIN; NUCLEAR-MAGNETIC-RESONANCE; O-SPECIFIC POLYSACCHARIDE; STRUCTURAL-ANALYSIS; WALL MANNAN; BIOPSY SPECIMENS; LIPOPOLYSACCHARIDES; ANTIGEN; YEAST; GENE |
|
abstract |
With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of alpha-(1 -> 6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both alpha- and beta-configurations: ->[6)-alpha-D-Manp-(1 -> 6](n)-alpha-D-Manp-(1 -> 6 R1 -> 2 R = alpha-D-Man; ->[2)-alpha-D-Man-(1](n)-> 2)-alpha-D-Man; ->[2)-alpha-D-Man-(1]n ->? In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host-microbial interactions and immunological modulation. (C) 2010 Elsevier Ltd. All rights reserved. |
|
publisher |
ELSEVIER SCI LTD |
|
issn |
0008-6215 |
|
year published |
2010 |
|
volume |
345 |
|
issue |
6 |
|
beginning page |
830 |
|
ending page |
838 |
|
digital object identifier (doi) |
10.1016/j.carres.2010.01.022 |
|
web of science category |
Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Organic |
|
subject category |
Biochemistry & Molecular Biology; Chemistry |
|
unique article identifier |
WOS:000277112800014
|
