Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells
authors Duarte, IF; Lamego, I; Marques, J; Marques, MPM; Blaise, BJ; Gil, AM
nationality International
journal JOURNAL OF PROTEOME RESEARCH
author keywords osteosarcoma; MG-63 cells; cisplatin; cancer; cancer therapy; metabonomics; NMR spectroscopy
keywords DOSE 2 GY; BREAST-CANCER; 3-DIMENSIONAL SPHEROIDS; IONIZING-RADIATION; MOBILE LIPIDS; K562 CELLS; IN-VITRO; H-1-NMR; SPECTROSCOPY; APOPTOSIS
abstract In the present study, H-1 HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
publisher AMER CHEMICAL SOC
issn 1535-3893
year published 2010
volume 9
issue 11
beginning page 5877
ending page 5886
digital object identifier (doi) 10.1021/pr100635n
web of science category Biochemical Research Methods
subject category Biochemistry & Molecular Biology
unique article identifier WOS:000283810500036
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journal impact factor 3.950
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