Extraction of Biomolecules Using Phosphonium-Based Ionic Liquids + K3PO4 Aqueous Biphasic Systems
authors Louros, CLS; Claudio, AFM; Neves, CMSS; Freire, MG; Marrucho, IM; Pauly, J; Coutinho, JAP
nationality International
journal INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
author keywords ionic liquids; aqueous two-phase systems; phase diagrams; partition coefficients
keywords AQUEOUS BIPHASIC SYSTEMS; MUTUAL SOLUBILITIES; 2-PHASE SYSTEMS; SALTING-OUT; PLUS WATER; PHASE-BEHAVIOR; PROTEINS; EQUILIBRIA; SEPARATION; SOLVENTS
abstract Aqueous biphasic systems (ABS) provide an alternative and efficient approach for the extraction, recovery and purification of biomolecules through their partitioning between two liquid aqueous phases. In this work, the ability of hydrophilic phosphonium-based ionic liquids (ILs) to form ABS with aqueous K3PO4 solutions was evaluated for the first time. Ternary phase diagrams, and respective tie-lines and tie-lines length, formed by distinct phosphonium-based ILs, water, and K3PO4 at 298 K, were measured and are reported. The studied phosphonium-based ILs have shown to be more effective in promoting ABS compared to the imidazolium-based counterparts with similar anions. Moreover, the extractive capability of such systems was assessed for distinct biomolecules (including amino acids, food colourants and alkaloids). Densities and viscosities of both aqueous phases, at the mass fraction compositions used for the biomolecules extraction, were also determined. The evaluated IL-based ABS have been shown to be prospective extraction media, particularly for hydrophobic biomolecules, with several advantages over conventional polymer-inorganic salt ABS.
publisher MDPI AG
issn 1422-0067
year published 2010
volume 11
issue 4
beginning page 1777
ending page 1791
digital object identifier (doi) 10.3390/ijms11041777
web of science category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
subject category Biochemistry & Molecular Biology; Chemistry
unique article identifier WOS:000277119800026
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journal impact factor 3.687
5 year journal impact factor 3.878
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