Effect of ionic liquids activation on laccase from Trametes versicolor: Enzymatic stability and activity
authors Tavares, APM; Pereira, JAN; Xavier, AMRB
nationality International
journal ENGINEERING IN LIFE SCIENCES
author keywords Activation of laccase; Ionic liquids; Michaelis-Menten kinetics; Thermal inactivation; Trametes versicolor
keywords HORSERADISH-PEROXIDASE; CATALYTIC-ACTIVITY; THERMAL-STABILITY; AQUEOUS-SOLUTIONS; BIOCATALYSIS; STABILIZATION; PERFORMANCE; SOLVENTS; OXIDASE; ACID
abstract The stability and activity of laccase from Trametes versicolor in two water-soluble ionic liquids (ILs), namely 1-butyl-3-methylimidazolium methyl sulfate, [bmim][MeSO4] and 1,3-dimethylimidazolium methyl sulfate, [mmim][MeSO4], were investigated in this study. Thermal inactivation of laccase was characterized in the presence of these both ILs and as expected first-order kinetics was followed. Inactivation rate constant (k), half-life time (t1/2), and energy of activation (Ea) were determined. Kinetics of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) oxidation by laccase in the presence of these ILs was studied and MichaelisMenten parameters were calculated. There is no enzymatic inactivation since the maximum reaction rate remained constant for IL concentrations up to 25%, and surprisingly, it was found that laccase was activated for concentrations of 35% of ILs, since the reaction rate increased 1.7 times.
publisher WILEY-BLACKWELL
issn 1618-0240
year published 2012
volume 12
issue 6
beginning page 648
ending page 655
digital object identifier (doi) 10.1002/elsc.201100203
web of science category Biotechnology & Applied Microbiology
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000312029600009
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journal impact factor 2.385
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