Diamond based biosensors: surface-fluid interaction issues
authors Neto, MA; Gomes, CMF; Silva, EL; Fernandes, AJS; Oliveira, FJ; Silva, RF
editors Luxton, R
nationality International
journal 2ND INTERNATIONAL CONFERENCE ON BIO-SENSING TECHNOLOGY
author keywords Diamond surfaces; boron doped; bioinertess; simulated body fluid; albumin
keywords THIN-FILMS
abstract The interaction of nanocrystalline (NCD) and microcrystalline (MCD) p-type semiconducting diamond surfaces with phosphate buffer saline (PBS), simulated body fluid (SBF) and bovine serum albumin (BSA) solutions under applied electric potentials is the main subject of this work. The effect of these fluids on the electrical resistivity of the diamond surface is evaluated. Furthermore, the interaction effects of these surfaces with BSA containing SBF solution during 30 days, is addressed. The electrical measurements have shown that the electrical current passing through the NCD samples is influenced by the fluid's ionic composition, whereas no variation was detected for the MCD samples. Both NCD and MCD surfaces favor the formation of a deposit layer after 30 days in contact with SBF+BSA solution. This deposit is mainly composed of Ca, P, Mg and O, containing also a significant concentration of BSA proteins. (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of the Institute of Bio-Sensing Technologies, UWE Bristol.
publisher ELSEVIER SCIENCE BV
issn 1876-6196
year published 2012
volume 6
beginning page 117
ending page 124
digital object identifier (doi) 10.1016/j.proche.2012.10.137
web of science category Biophysics; Chemistry, Physical
subject category Biophysics; Chemistry
unique article identifier WOS:000313216900015

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