CotA laccase-ABTS/hydrogen peroxide system: An efficient approach to produce active and decolorized chitosan-genipin films
authors Goncalves, I; Nunes, C; Mendes, S; Martins, LO; Ferreira, P; Coimbra, MA
nationality International
journal CARBOHYDRATE POLYMERS
author keywords Chitosan-genipin films; Decolorization; Laccase; Mediator; Hydrogen peroxide; Active materials
keywords BOVINE SERUM-ALBUMIN; CORN FIBER GUM; ANTIOXIDANT ACTIVITY; HYDROGEN-PEROXIDE; BACILLUS-SUBTILIS; STABILITY; BACTERIAL; MEDIATOR; CYTOCOMPATIBILITY; GLYCEROL
abstract Chitosan-genipin films present a bluish-green color due to the conjugated double bonds formed when monomeric or dimeric genipin residues bridge chitosan. This phenomenon limits their use when colorless materials are required. In this work, a two-step oxidation strategy was developed aiming to remove color from chitosan-genipin films while preserving their functional properties. A combined system using the recombinant CotA laccase from Bacillus subtilis mediated by 2,2'-azinobis-(3-ethylbenzothiazoline6-sulphonic acid) (ABTS) followed by a H2O2 oxidation step was settled. ABTS boosted the laccase performance resulting in light brown chitosan-genipin films that were further decolorized upon immersion in a 5% H2O2 solution at pH 11.0 and 40 degrees C for 30 min. The applied methodology leads to films that sustain both acidic stability and antioxidant capacity of the pristine films. Overall, the combined CotA laccase-ABTS/hydrogen peroxide developed methodology efficiently produce active and decolorized chitosan-genipin films with potential for application as eco-friendly transparent material which may have application as food packaging. (C) 2017 Elsevier Ltd. All rights reserved.
publisher ELSEVIER SCI LTD
issn 0144-8617
year published 2017
volume 175
beginning page 628
ending page 635
digital object identifier (doi) 10.1016/j.carbpol.2017.08.029
web of science category Chemistry, Applied; Chemistry, Organic; Polymer Science
subject category Chemistry; Polymer Science
unique article identifier WOS:000411112500072
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journal analysis (jcr 2017):
journal impact factor 5.158
5 year journal impact factor 5.326
category normalized journal impact factor percentile 93.599
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