Biological control of Aeromonas salmonicida infection in juvenile Senegalese sole (Solea senegalensis) with Phage AS-A
authors Moreirinha, C; Silva, YJ; Pereira, C; Costa, L; Rocha, RJM; Cunha, A; Gomes, NCM; Calado, R; Almeida, A
nationality International
author keywords Disease; Fish pathogenic bacteria; Bacterial control; Phage therapy; Aeromonas salmonicida; Furunculosis; Solea juveniles
abstract One of the major sources of financial loss for the fish farming industry is the occurrence of infections by pathogenic bacteria, especially multidrug-resistant variants. This problem is most prominent during the early stages of fish development and is difficult to address with traditional antibiotic treatment or vaccination. In this way, alternative environmentally-friendly biological strategies to control bacterial infections need to be implemented. Under this scenario, phage therapy appears as a useful and flexible tool for the inactivation of bacterial pathogens in aquaculture. The aim of this study was to test the efficacy of phage therapy to inactivate Aeromonas salmonicida, the causative agent of furunculosis, a fish disease characterized by high mortality and morbidity. In order to achieve this goal, a new phage was isolated, characterized and tested in artificially-infected Solea senegalensis juveniles and in batch bacterial cultures. Results showed that after 6 h of treatment the phage inhibited the growth of A. salmonicida both in batch cultures and seawater in the presence of fish juveniles (approximate to 4 and 2.5 Log PFU mL(-1), respectively). After 72 h, fish juveniles treated with phages after exposure to A. salmonicida showed no mortality, contrarily to fishes that were only exposed to the bacterium, which presented a mortality of 36%. This result indicated that phage treatment was effective. In general, a limited regrowth of resistant cells and absence of lysogeny conversion were observed. No significant impact of phage inoculation on natural bacterial communities of aquaculture waterwas detected. However, the bacterial community associated with fish intestinal tractwas moderately affected by the addition of the phage. Interestingly, the differences were not significant when the phage was added in the presence of the host bacteria. Taking this into account, this study provides evidences that the tested phage can be effective and safe against furunculosis during the production of juvenile fish. Statement of relevance A major source of financial loss for the fish-farming industry is the occurrence of bacterial infections. This problem is most prominent in early stages of fish development, and is difficult to address with antibiotics/vaccination. Alternative environmentally-friendly strategies to control bacterial infections are needed. This study indicates that phages can be effective and safe against furunculosis during fish juvenile production. (C) 2015 Elsevier B.V. All rights reserved.
issn 0044-8486
year published 2016
volume 450
beginning page 225
ending page 233
digital object identifier (doi) 10.1016/j.aquaculture.2015.07.025
web of science category Fisheries; Marine & Freshwater Biology
subject category Fisheries; Marine & Freshwater Biology
unique article identifier WOS:000364854000031
  ciceco authors
  impact metrics
journal analysis (jcr 2017):
journal impact factor 2.710
5 year journal impact factor 2.978
category normalized journal impact factor percentile 83.884
dimensions (citation analysis):
altmetrics (social interaction):