|
authors |
Leite, AJ; Oliveira, MB; Caridade, SG; Mano, JF |
|
nationality |
International |
|
journal |
ADVANCED FUNCTIONAL MATERIALS |
|
author keywords |
array-based screening; combinatorial biomaterials screening; flat platforms; high-throughput screening; nanocomposites; nondestructive image analysis |
|
keywords |
STEM-CELL FATE; TISSUE-ENGINEERING APPLICATIONS; GLASS-CERAMIC NANOPARTICLES; DYNAMIC-MECHANICAL ANALYSIS; IN-VITRO CHARACTERIZATION; HYALURONIC-ACID; BIOACTIVE GLASS; HIGH-THROUGHPUT; REGENERATIVE MEDICINE; COMPOSITE SCAFFOLDS |
|
abstract |
Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments. |
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publisher |
WILEY-V C H VERLAG GMBH |
|
issn |
1616-301X |
|
isbn |
1616-3028 |
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year published |
2017 |
|
volume |
27 |
|
issue |
28 |
|
digital object identifier (doi) |
10.1002/adfm.201701219 |
|
web of science category |
Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter |
|
subject category |
Chemistry; Science & Technology - Other Topics; Materials Science; Physics |
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unique article identifier |
WOS:000406183100015
|
