Gold nanorods induce early embryonic developmental delay and lethality in zebrafish (Danio rerio)
authors Mesquita, B; Lopes, I; Silva, S; Bessa, MJ; Starykevich, M; Carneiro, J; Galvao, TLP; Ferreira, MGS; Tedim, J; Teixeira, JP; Fraga, S
nationality International
journal JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
keywords DAPHNIA-MAGNA; NANOPARTICLE UPTAKE; SURFACE-CHEMISTRY; DRUG-DELIVERY; MODEL SYSTEM; IN-VITRO; TOXICITY; CELLS; EXPOSURE; SHAPE
abstract Due to their unique electronic and optical features, gold nanoparticles (AuNP) have received a great deal of attention for application in different fields such as catalysis, electronics, and biomedicine. The large-volume manufacturing predicted for future decades and the inevitable release of these substances into the environment necessitated an assessment of potential adverse human and ecological risks due to exposure to AuNP. Accordingly, this study aimed to examine the acute and developmental toxicity attributed to a commercial suspension of Au nanorods stabilized with cetyltrimethylammonium bromide (CTAB-AuNR) using early embryonic stages of zebrafish (Danio rerio), a well-established model in ecotoxicology. Zebrafish embryos were exposed to CTAB-AuNR (0-150 mu g/L) to determine for developmental assessment until 96 hr post fertilization (hpf) and lethality. Uptake of CTAB-AuNR by embryos and nanoparticles potential to induce DNA damage was also measured at 48 and 96 hpf. Analysis of the concentration-response curves with cumulative mortality at 96 hpf revealed a median lethal concentration (LC50,96h) of 110.2 mu g/L. At sublethal concentrations, CTAB-AuNR suspensions were found to produce developmental abnormalities such as tail deformities, pericardial edema, decreased body length, and delayed eye, head, and tail elongation development. Further, less than 1% of the initial concentration of CTAB-AuNR present in the exposure media was internalized by zebrafish embryos prior to (48 hpf) and after hatching (96 hpf). In addition, no marked DNA damage was detected in embryos after exposure to CTAB-AuNR. Overall, CTAB-AuNR suspensions produced lethal and sublethal effects on zebrafish embryos with possible repercussions in fitness of adult stages. However, these results foresee a low risk for fish since the observed effects occurred at concentrations above the levels expected to find in the aquatic environment.
publisher TAYLOR & FRANCIS INC
issn 1528-7394
year published 2017
volume 80
issue 13-15
beginning page 672
ending page 687
digital object identifier (doi) 10.1080/15287394.2017.1331597
web of science category Environmental Sciences; Public, Environmental & Occupational Health; Toxicology
subject category Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology
unique article identifier WOS:000416344500009
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journal impact factor (jcr 2016): 2.731
5 year journal impact factor (jcr 2016): 2.398
category normalized journal impact factor percentile (jcr 2016): 67.895
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