Tuning lysozyme nanofibers dimensions using deep eutectic solvents for improved reinforcement ability
authors Silva, NHCS; Vilela, C; Pinto, RJB; Martins, MA; Marrucho, IM; Freire, CSR
nationality International
journal INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
author keywords Aspect-ratio; Deep eutectic solvents; Fibrillation; Lysozyme nanofibers; Reinforced pullulan films
keywords EGG-WHITE LYSOZYME; AMYLOID FIBRILS; TRANSPARENT FILMS; IONIC LIQUIDS; PULLULAN; PROTEIN; AMYLOIDOGENESIS; AGGREGATION; COMPOSITES; ACIDS
abstract Deep eutectic solvents (DESs), a novel generation of solvents, have recently been described as efficient and timesaving fibrillation agents for proteins. In this context, the present work aims at assessing the effect of the hydrogen bond donor (HBD) of cholinium chloride ([Ch]Cl):carboxylic acid based DESs on the dimensions (length and width) of lysozyme nanofibers (LNFs). Mono-, di- and tri-carboxylic acids (acetic, lactic, levulinic, malic and citric acids) were used to prepare different DES formulations, which were successfully used on the fibrillation of lysozyme. The results showed that the carboxylic acid (i.e. the HBD) plays an important role on the fibrillation efficiency and on the length of the ensuing LNFs with aspect-ratios always higher than those obtained by fibrillation with [Ch]Cl. The longest LNFs were obtained using lactic acid as the HBD with an average length of 1004 +/- 334nm and width of 31.8 +/- 6.8nm, and thus an aspect-ratio of ca. 32. The potential of these protein nanofibers as reinforcing additives was evaluated by preparing pullulan (PL)-based nanocomposite films containing 5% LNFs with different aspect-ratios, resulting in highly homogenous and transparent films with improved mechanical performance. (C) 2018 Published by Elsevier B.V.
publisher ELSEVIER SCIENCE BV
issn 0141-8130
year published 2018
volume 115
beginning page 518
ending page 527
digital object identifier (doi) 10.1016/j.ijbiomac.2018.03.150
web of science category Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science
subject category Biochemistry & Molecular Biology; Chemistry; Polymer Science
unique article identifier WOS:000438662500059
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journal impact factor (jcr 2016): 3.671
5 year journal impact factor (jcr 2016): 3.657
category normalized journal impact factor percentile (jcr 2016): 81.060
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