Enhanced biocatalytic sustainability of laccase by immobilization on functionalized carbon nanotubes/polysulfone membranes
authors Costa, JB; Lima, MJ; Sampaio, MJ; Neves, MC; Faria, JL; Morales-Torres, S; Tavares, APM; Silva, CG
nationality International
journal CHEMICAL ENGINEERING JOURNAL
author keywords Multi-walled carbon nanotubes (CNTs); Chemical functionalization; Laccase; Immobilization; CNTs-based polysulfone membranes; Wastewater treatment
keywords REACTIVE TEXTILE DYES; ENZYME IMMOBILIZATION; POLYSULFONE MEMBRANES; COMMERCIAL LACCASE; FUNGAL LACCASES; WASTE-WATER; NANOTUBES; OXIDATION; DEGRADATION; SURFACE
abstract Chemically functionalized multi-walled carbon nanotubes (CNTs) are used as carriers for laccase immobilization. In this work, CNTs were modified using different approaches with a combination of methods involving hydrothermal oxidation with nitric acid, treatment with 3-aminopropyltriethoxysilane, glutaraldehyde, N-ethyl-N-(3-(dimethyl-amino)- propyl) carbodiimide hydrochloride and N-hydroxysuccinimide. The enzyme immobilization efficiency and recovered activity were evaluated towards 2,2'-azino-bis(3-ethylbenzathiazoline-6-sulfonic acid) biocatalytic oxidation. The best compromise between immobilization efficiency and recovered activity was obtained using the CNTs functionalized with 0.3M HNO3, treated with N-ethyl-N-(3-(dimethylamino) propyl) carbodiimide hydrochloride and N-hydroxysuccinimide. This catalyst also showed the best thermal stability (at 50 and 60 degrees C). The bioconjugate based on this material was characterized by vibrational spectroscopies (FTIR and Raman) and by N-2 adsorption. The results from reutilization tests showed that laccase activity was kept above 65% of its initial value after five consecutive cycles of reuse. The biocatalytic performance of the immobilized enzyme was evaluated for the degradation of a mixture of phenolic compounds in water containing phenol, resorcinol, 4-methoxyphenol and 4-chlorophenol. As means of cost efficient to enzyme reutilization, laccase was immobilized over polysulfone membranes blended with the functionalized CNTs and studied in the degradation of 4-methoxyphenol.
publisher ELSEVIER SCIENCE SA
issn 1385-8947
year published 2019
volume 355
beginning page 974
ending page 985
digital object identifier (doi) 10.1016/j.cej.2018.08.178
web of science category Engineering, Environmental; Engineering, Chemical
subject category Engineering
unique article identifier WOS:000445416900092
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