Doping beta-TCP as a Strategy for Enhancing the Regenerative Potential of Composite beta-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats

authors	Ferreira, MM; Brito, AF; Brazete, D; Pereira, IC; Carrilho, E; Abrantes, AM; Pires, AS; Aguiar, MJ; Carvalho, L; Botelho, MF; Ferreira, JMF
nationality	International
journal	MATERIALS
author keywords	composite bone grafts; calcium phosphates; implants; dentistry; biomedical engineering; osteogenesis
keywords	TRICALCIUM PHOSPHATE; IN-VITRO; STRONTIUM RANELATE; ZINC; CERAMICS; MECHANISMS; SCAFFOLDS; BEHAVIOR; 45S5; BIODEGRADATION
abstract	The present work aims at evaluating the potential gains derived from partially replacing calcium in resorbable beta-tricalcium phosphate (beta-TCP) by two different molar percentages of strontium (5, 10) and zinc (1, 2), concomitantly with a fixed molar percentage (0.5) of manganese. Synthetic granular composite bone filling grafts consisting of doped beta-TCP and an alkali-free bioactive glass were prepared and implanted in similar to 4 mm diameter bone defects drilled in the calvaria of Wistar rats used as animal models. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Non-manipulated bone was used as positive control, while empty defects were used as a negative control group. The von Kossa staining revealed an enhanced new bone formation with increasing doping levels, supporting the therapeutic effects exerted by the doping elements. The percentage of newly formed bone was similar when the defects were filled with autologous bone, BG (previous results) or 3TCP2/7BG, which indicates that the latter two are excellent candidates for replacement of autologous bone as bone regeneration material. This finding confirms that doping with suitable doses of therapeutic ions is a good strategy towards transposing the bone graft materials to biomedical applications in humans.
publisher	MDPI
issn	1996-1944
year published	2019
volume	12
issue	1
digital object identifier (doi)	10.3390/ma12010004
web of science category	Materials Science, Multidisciplinary
subject category	Materials Science
unique article identifier	WOS:000456410200004