Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes
authors Pereira, P; Barreira, M; Cruz, C; Tomas, J; Luis, A; Pedro, AQ; Queiroz, JA; Sousa, F
nationality International
journal PHARMACEUTICALS
author keywords blood-brain barrier; chitosan; drug delivery system; lactoferrin; polyethyleneimine; recombinant miRNA
keywords TRANSFERRIN RECEPTOR; ALZHEIMERS-DISEASE; ENDOTHELIAL-CELLS; IN-VITRO; DRUG-DELIVERY; BARRIER; CHITOSAN; NANOPARTICLES; TRANSCYTOSIS; LIPOSOMES
abstract The efficacy of brain therapeutics is largely hampered by the presence of the blood-brain barrier (BBB), mainly due to the failure of most (bio) pharmaceuticals to cross it. Accordingly, this study aims to develop nanocarriers for targeted delivery of recombinant precursor microRNA (pre-miR-29b), foreseeing a decrease in the expression of the BACE1 protein, with potential implications in Alzheimer's disease (AD) treatment. Stearic acid (SA) and lactoferrin (Lf) were successfully exploited as brain-targeting ligands to modify cationic polymers (chitosan (CS) or polyethyleneimine (PEI)), and its BBB penetration behavior was evaluated. The intracellular uptake of the dual-targeting drug delivery systems by neuronal cell models, as well as the gene silencing efficiency of recombinant pre-miR-29b, was analyzed in vitro. Labeled pre-miR-29b-CS/PEI-SA-Lf systems showed very strong fluorescence in the cytoplasm and nucleus of RBE4 cells, being verified the delivery of pre-miR-29b to neuronal cells after 1 h transfection. The experiment of transport across the BBB showed that CS-SA-Lf delivered 65% of recombinant pre-miR-29b in a period of 4 h, a significantly higher transport ratio than the 42% found for PEI-SA-Lf in the same time frame. Overall, a novel procedure for the dual targeting of DDS is disclosed, opening new perspectives in nanomedicines delivery, whereby a novel drug delivery system harvests the merits and properties of the different immobilized ligands.
publisher MDPI
isbn 1424-8247
year published 2020
volume 13
issue 10
digital object identifier (doi) 10.3390/ph13100314
web of science category Pharmacology & Pharmacy
subject category Pharmacology & Pharmacy
unique article identifier WOS:000587435900001
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journal analysis (jcr 2019):
journal impact factor 4.286
5 year journal impact factor Not Available
category normalized journal impact factor percentile 82.037
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