|
authors |
Freire, F; Romao, MJ; Macedo, AL; Aveiro, SS; Goodfellow, BJ; Carvalho, AL |
|
nationality |
International |
|
journal |
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
|
keywords |
MOLECULAR-REPLACEMENT; P22HBP; FAMILY |
|
abstract |
Human SOUL (hSOUL) is a 23 kDa haem-binding protein that was first identified as the PP(23) protein isolated from human full-term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P6(4)22, with unit-cell parameters a = b = 145, c = 60 angstrom and one protein molecule in the asymmetric unit. X-ray diffraction data were collected to 3.5 angstrom resolution at the ESRF. A preliminary model of the three-dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva), obtained by solution NMR, as search models. |
|
publisher |
WILEY-BLACKWELL PUBLISHING, INC |
|
issn |
1744-3091 |
|
year published |
2009 |
|
volume |
65 |
|
beginning page |
723 |
|
ending page |
726 |
|
digital object identifier (doi) |
10.1107/S174430910902291X |
|
web of science category |
Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography |
|
subject category |
Biochemistry & Molecular Biology; Biophysics; Crystallography |
|
unique article identifier |
WOS:000267530000018
|
