Clinical Proteomics
authors Cova, Marta; Oliveira-Silva, Rui; Ferreira, Jose Alexandre; Ferreira, Rita; Amado, Francisco; Daniel-da-Silva, Ana Luisa; Vitorino, Rui
chapter title Glycoprotein Enrichment Method Using a Selective Magnetic Nano-Probe Platform (MNP) Functionalized with Lectins.
edition Methods in molecular biology (Clifton, N.J.) 2nd edition
nationality United States
abstract Protein post-translational modifications (PTMs) have increasingly become a research field of incredible importance to fully understand the regulation of biological processes in health and disease. Among PTMs, glycosylation is one of the most studied for which contributed the development and improvement of enrichment techniques. Nowadays, glycoprotein enrichment methods are based on lectin affinity, covalent interactions, and hydrophilic interaction liquid chromatography (HILIC). Nonetheless, the nanotechnology era has fetched new methods to enrich glycoproteins from complex samples as human biological fluids. For instance, magnetic nanoparticles (MNPs) are being used as an interesting enrichment approach allowing a better characterization of glycoproteins and glycopeptides.In this chapter, we describe an enrichment method based on MNPs functionalized with lectins (Concavalin A, wheat germ agglutinin, and Maackia amurensis lectin) to enrich specific sets of glycoproteins from biological fluids. Moreover, it is proposed a bioinformatic strategy to deal with data retrieved from mass spectrometry analysis of enriched samples aiming the identification of relevant biological processes modulated by a given stimuli and, ultimately, of new biomarkers for disease screening/management.
issn 1940-6029
volume 1243
beginning page 83
ending page 100
book digital object identifier (doi) 10.1007/978-1-4939-1872-0_5
subject category Protein enrichment
link http://link.springer.com/protocol/10.1007%2F978-1-4939-1872-0_5
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