Interdisciplinary Studies on Environmental Chemistry - Biological Responses to Contaminants
authors Nogueira P, Pacheco M, Pereira ML, Mendo S, Rotchell M
editors N Hamamura, S Suzuki, S Mendo, CM Barroso, H Iwata and S Tanabe
chapter title Novel Potential Molecular Biomarkers of Aquatic Contamination in Dicentrarchus labrax and Liza aurata
nationality International
author keywords sediment; urban channels; metals; enrichment factors
abstract The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using a combination of both traditional and novel biomarkers. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a down-regulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses.
publisher TERRAPUB
isbn 978-4887041523
year published 2010
volume 3
beginning page 127
ending page 138


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