Ana Maria Rebelo Barreto Xavier
  Assistant Professor  
   
  phone: 234 370 716  
  extension: 33533  
  e-mail: abx@ua.pt  
  department: Chemistry  
  office: 29.1.32  
   
  group: 4 - biorefineries, biobased materials and recycling
 
  line: 3 - sustainability and health
 
  direct url: http://www.ciceco.ua.pt/AnaXavier  
   
Short CV

Degree (“Licenciatura”) in Aplicated Chemistry - Biotechnology, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal, 23/07/87.
PhD in Biological Engineering, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa (FCT-UNL)under the supervision of Professor Manuel Carrondo. Thesis: “Adsorção de ácido lático e sua produção em fermentador com recirculação celular em membranas de ultrafiltração” (1996). Graduation in Chemistry - Biotechnology, (FCT-UNL).

1993: Assistant (“Assistente Estagiária”), University of Aveiro.

Since 1996: Assistant (“Auxiliar”) Professor, University of Aveiro

 

Scientific Interests

Ligninolytic activity for bleaching processes of pulp and paper industry.
Ligninolytic activity for pulp and paper effluent cleaning processes.
Biorefinary - Potentialities of hardwood spent sulfite liquor.
Imobilized yeasts for wine production. Bioethanol and biopolymers production.
Ligninolytic activity for bleaching processes of pulp and paper industry.
Ligninolytic activity for pulp and paper effluent cleaning processes.
Biorefinary - Potentialities of hardwood spent sulfite liquor.
Imobilized yeasts for wine production. Bioethanol and biopolymers production.

Present Research Group

Luísa Serafim
Dmytri Evtyugin

Teaching Activity

Undergraduate courses: Food Biotechnology, Microbial Biotechnology, Biocatalysts, Unit Operations in Food Processing, Mass and Energetic Balances and Mass Transfer, General Chemical Engineering Chemistry and Biochemistry Pratical classes.

 

MSc Thesis supervision
“Estudo da actividade lenhinolítica do Trametes versicolor” with co-orientation – 2000
“Estudo da biodegradação da lenhina pelo fungo Trametes versicolor“ with co-orientation – 2001
"Tratamento de efluente de produção de pasta Kraft por Trametes versicolor" with co-orientation - 2007
“Produção de bioetanol a partir de licor de cozimento ao sulfito ácido” with co-orientation, 2008, Theses premiated with first prize of ChemPor Jovem 2008
“Produção de proteína microbiana a partir de licor de cozimento ao sulfito ácido” with co-orientation - 2008
“Optimização de um fermentador de leito fixo para desacidificação biológica de mostos e vinhos" in PROENOL with co-orientation, 2008
“Projecto de optimização do tratamento biológico, com o sistema BioAmp GT, em águas residuais”– in PROQUICHEME, with co-orientation, 2008
“Fermentação contínua de mosto com leveduras imobilizadas em alginato" in PROENOL with co-orientation - 2009
"Tratamento biológico de efluente de produção kraft por Phlebia rufa", with co-orientation - 2009
"Produção, isolamento e caracterização química do exopolissacarídeo do fungo da prodridão branca da madeira Tramentes versicolor", with co-orientation - 2009
"Estudo da estabilidade térmica e bárica da levedura Pichia stipitis", with co-orientation - 2009"
"Bioproccessing of Spent Sulphite Liquor for Bioethanol and Single Cell Protein production", with co-orientation -2010
"Biorefinery: valorisation of pulp and paper industry side-products for bioplastics production", with co-orientation -2010
"Estudo da estabilidade, actividade e cinética enzimática da lacase em líquidos iónicos", with co-orientation - 2010
"Cinética de Produção de Etanol por Pichia stipitis" -", with co-orientation - 2011
“Estudo e optimização do funcionamento de uma ETAR industrial” –with co-orientation - 2012
“Produção de espumante pelo Método Charmat com leveduras imobilizadas” -with co-orientation - 2012
"Produção de bioetanol a partir de SSLs: S. stipitis vs S. cerevisae" -with co-orientation - 2012

"Certificação de processos na área da biotecnologia alimentar" -with co-orientation - 2013
 “Produção de bioetanol: Efeito da concentração da fonte de carbono” -with co-orientation - 2013
“O efeito de acumulação de piruvato em transaminações com levedura” with co-orientation 2013

“Caraterização de Leveduras Imobilizadas por Citometria de Fluxo”-with co-orientation - 2013

 “Processos de Fracionamento para a Valorização da Palha de Milho”-with co-orientation - 2013
“Estabilização tartárica do vinho por adição da carboximetilcelulose”-with co-orientation - 2014
“Estudo da hidrólise da pasta kraft com diferentes enzimas”-with co-orientation - 2014
“Imobilização de lacase em nanopartículas de magnetite”-with co-orientation - 2014
“Desafios na produção de etanol a partir de biomassa lenhocelulósica”-with co-orientation - 2014
“Macroalgas como matéria-prima para a cultura de bactérias marinhas”with co-orientation - 2015
“Estudo da imobilização da lacase em nanopartículas magnéticas e sua aplicação”with co-orientation-2015
“Estudo do comportamento fermentativo de Actinobacillus succinogenes para a produção de ácido succínico e ácido lático” -with co-orientation - 2015
“Estudo do efeito do arejamento na fermentação de Scheffersomyces stipitis”-with co-orientation - 2015
“Eco-engenharia de culturas microbianas produtoras de bioplásticos” -with co-orientation - 2015
Rita Daniela Marques Sousa “Acidificação de resíduos industriais para obter substratos microbianos” -with co-orientation - 2015
Ana Cristina Abreu Capitão “Otimização do processo de Dry-Hopping na Casa da Cerveja” -with co-orientation - 2016
“Produção de Biocombustível de 2ª Geração:  Obtenção de Bioetanol a partir de Cardo”-with co-orientation - 2016
“Mixotrophic fermentation for butanol production”-with co-orientation - 2017
“ Tratamento enzimático de pasta kraft de Eucalyptus glóbulos como estratégia inovadora no processo de branqueamento” - -with co-orientation - 2017
“Avaliação de soluções para o scuffing em garrafas de tara retornável”-with co-orientation - 2018
“Produção de Bioetanol de 2ª Geração numa lógica de Economia Circular” with co-orientation 2018
“Produção de Bioetanol numa lógica de Economia Circular”-with co-orientation - 2018
Joshua André Margarido Anjos “Estudo da produção de Kefir e Kefirano”--with co-orientation - 2018
 
 

 2 PhD theses supervised
“Produção de lacase para potencial aplicação como oxidante na indústria papeleira” with co-orientation –2006, Theses premiated with an honour mention by 2006-CUF Premium


“Second generation bioethanol production from a sub-product of pulping industry” with co-orientation 2013.



Main present collaborators

Luísa Serafim
Dmytri Evtyugin
Inês Portugal
Jorge Saraiva

Present projects
“Valorização de produtos agro-industriais de baixo valor” (1997-2000) - University of Aveiro - Coordinator of an internal project.


POCTI/EQU/48489/2002
"Study of Pulp and Paper Industry effluent cleaning by lignolytic cultures of Trametes versicolor"(2003-2006)- Coordinator.

"Biorefinery – Potentialities of hardwood spent sulfite liquor" - financiado pela Indústria Papeleira
Book Chapters

Serafim L.S., Xavier A.M.R.B., Lemos P.C. (2018) Storage of Hydrophobic Polymers in Bacteria. In: Geiger O. (eds) Biogenesis of Fatty Acids, Lipids and Membranes. Handbook of Hydrocarbon and Lipid Microbiology. Springer, Cham.  Online ISBN 978-3-319-43676-0. DOI https://doi.org/10.1007/978-3-319-43676-0_33-1  
 
Ana P M Tavares, Cláudia G Silva and Ana M R B Xavier, Laccase properties, reaction mechanisms and applications: an overview in Laccase, Applications, Investigations and Insights, Edited by Amanda Harris in Biochemistry Research Trends, Nova Publishers, New York, 1st chap, 1-26, 2017. ISBN: 978-1-53610-582-7.
 
Ana P.M. Tavares and Ana M.R.B. Xavier (2014), Application of Oxidative Enzymes in the Treatment of Industrial Effluents, chapter 7 in Biotechnology Vol. 11:  Biodegradation and Biorremediation, 193–214. Studium Press LLC, Houston, TX, USA. ISBN: 1-62699-026-1.
 
Ana P.M. Tavares, Ana M.R.B. Xavier and Dmitry Evtuguin (2014), Biotechnology Applications in Pulp and Paper Industry, chapter 22 in Biotechnology Vol.12: Bioprocess Engineering, 561–581. Studium Press LLC, Houston, TX, USA. ISBN: 1-62699-027-1.
 
Daniel L. A. Fernandes, Susana R. Pereira, Luísa S. Serafim, Dmitry Evtuguin and Ana M.R.B.Xavier (2012) Second Generation Bioethanol from Lignocellulosics: Processing of Hardwood Sulphite Spent Liquor, In Bioethanol, Marco A. P. Lima (Ed.), InTech, . InTech, Rijeka, Croatia pp. 123-152. ISBN 978-953-51-0008-9.

Selected Publications

Tiago M. Henriques; Susana R. Pereira; Luísa S. Serafim; Ana M.R.B. Xavier, Two-Stage Aeration Fermentation Strategy to Improve Bioethanol Production by Scheffersomyces stipitis. Fermentation 4, 97. 2018. https://doi.org/10.3390/fermentation4040097

Maria C.Fernandes, Miguel D.Ferro, Ana F.C.Paulino, Humberto T.Chaves, Dmitry V. Evtuguin, Ana M.R.B.Xavier,Comparative study on hydrolysis and bioethanol production from cardoon and rockrose pretreated by dilute acid hydrolysis. Industrial Crops and Products, 111, 633–641, 2018. https://doi.org/10.1016/j.indcrop.2017.11.037

Ana P.M. Tavares, Susana R. Pereira, and Ana M.R.B. Xavier, Biotechnological applications of Trametes versicolor and their enzymes, Current Biotechnology, 2017, 6, 78-88. DOI: 10.2174/2211550105666160510113212

Cristiano C. S. Fortes, Ana L Daniel-da-Silva, Ana P.M. Tavares, Ana M.R.B. Xavier, Optimization of enzyme immobilization on functionalized magnetic nanoparticles for laccase biocatalytic reactions. Chemical Engineering & Processing: Process Intensification, 117,1-8, 2017 https://doi.org/10.1016/j.cep.2017.03.009 
Raquel A. Fernandes, Ana L Daniel-da-Silva, Ana P.M. Tavares, Ana M.R.B. Xavier, EDTA-Cu (II) chelating magnetic nanoparticles as a support for laccase immobilization. Chemical Engineering Science 158 (2017) 599–605. http://dx.doi.org/10.1016/j.ces.2016.11.011  
 
A.M.R.B. Xavier, A.P.M.Tavares, A.L. Daniel-da-Silva, C. Fortes, Optimisation of laccase immobilization on modified magnetic nanoparticles for biocatalytic reactions.New Biotechnology, 33, S 15, 2016 https://doi.org/10.1016/j.nbt.2016.06.777 
Miguel D. Ferro, Maria C. Fernandes, Ana F.C. Paulino, Sónia O. Prozil, Janis Gravitis, Dmitry V. Evtuguin, Ana M.R.B. Xavier, Bioethanol production from steam explosion pretreated and alkali extracted Cistus ladanifer (rockrose). Biochemical Engineering Journal, 104, 98-105, 2015. doi:10.1016/j.bej.2015.04.009

Maria C. Fernandes, Miguel D. Ferro, Ana F.C. Paulino, Joana A.S. Mendes, Janis Gravitis, Dmitry V. Evtuguin, Ana M.R.B. Xavier, Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion. Bioresource Technology 186, 309–315, 2015 https://doi.org/10.1016/j.biortech.2015.03.037

S.R. Pereira, V.Sànchez i Nogué, C.J R Frazão, L. S. Serafim, M.F.Gorwa-Grauslund, A.M R B Xavier, Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering. Biotechnology for Biofuels 8, 50, 2015. http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0234-y

D. Portugal-Nunes, V.Sànchez i Nogué, S.R Pereira, S.C.Craveiro, A.J. Calado, A.M.R.B.Xavier, Effect of cell immobilization and pH on Scheffersomyces stipitis growth and fermentation capacity in rich and inhibitory media. Bioresources and Bioprocessing 2,13, 2015 https://link.springer.com/article/10.1186/s40643-015-0042-z

Cláudio J. R. Frazão, Nuno H. C. Silva, Carmen S. R. Freire, Armando J. D. Silvestre, Ana M. R. B. Xavier, Ana P. M. Tavares, Bacterial cellulose as carrier for immobilization of laccase. Optimization and characterization. Engineering in Life Sciences, 14, 500–508, 2014. http://onlinelibrary.wiley.com/doi/10.1002/elsc.201400054/abstract;jsessionid=0D933233F72200230AF0C03649790AE6.f02t01

Ana P. M. Tavares, Rui P. Silva, António L. Amaral, Eugénio C. Ferreira, Ana M.R.B. Xavier. Image analysis technique as a tool to identify morphological changes in Trametes versicolor pellets according to exopolysaccharide or laccase production. Applied Biochemistry and Biotechnology, 172 (4): 2132-2142, 2014. https://link.springer.com/article/10.1007%2Fs12010-013-0675-3

Joana A.S. Mendes, Ana M.R.B. Xavier, Dmitry V. Evtuguin, Luísa P.C. Lopes. Integrated utilization of grape skins from white grape pomaces. Industrial Crops and Products 49: 286– 291, 2013.

Pereira SR, Nunes DJP, Evtuguin DV, Serafim LS, Xavier AMRB. Advances in ethanol production from hardwood spent sulphite liquors. Process Biochemistry 48: 272–282, 2013.

Ana P.M. Tavares, Juliana A. N. Pereira, Ana M.R.B. Xavier, Effect of Ionic Liquids activation on laccase from Trametes versicolor: enzymatic stability and activity, Engineering in Life Sciences, 12 (5) 1–8, 2012.

Daniel L. A. Fernandes, Carlos M. Silva, Ana M.R.B.Xavier,  Dmitry Evtuguin, Fractionation of
Sulphite Spent Liquor Using Ion Exchange Resins for Biochemical Processing, Journal of Biotechnology, 162, 415– 421, 2012.

Jorge A. Saraiva, Ana P. M. Tavares, Ana M. R. B.Xavier, Effect of the Inducers Veratryl Alcohol, Xylidine,and Ligninosulphonates on Activity and Thermal Stability and Inactivation Kinetics of Laccase from Trametes versicolor, Appl Biochem Biotechnol, 167:685–693, 2012.

S. R. Pereira, Š. Ivanuša, D.V. Evtuguin, L. S. Serafim, A. M.R.B. Xavier, Biological treatment of eucalypt spent sulphite liquors: A way to boost the production of second generation bioethanol. Bioresource Technology 103 131–135, 2012.

Portugal, I.,  Ribeiro, S. C., Xavier, A.M.R.B., Centeno, F. , Strehaiano, P. , Immobilised yeast grape must deacidification in a recycle fixed bed reactor.International Journal of Food Science and Technology  46, 284–289, 2011.

Xavier, A. M. R. B., Correia, M. F., Pereira, S. R., and Evtyugin, D. V., Second-generation bioethanol from eucalypt sulphite spent liquor. Bioresource Technology, 101: 2755–2761, 2010.

A.M.R.B. Xavier, A.P.M. Tavares, M.S.M. Agapito, D.V. Evtuguin, Sequential batch reactor for the kraft pulp mill effluent treatment with Trametes versicolor, Journal of Chemical Technology and Biotechnology, 83: 1602-1608, 2008.

Silva C. M. ; Neves P. S. ; Da Silva F. A. ; Xavier A. M. R. B. ; Eusébio M. F. J. Tangential Ultrafiltration of Aqueous Saccharomyces cerevisiae Suspensions. Journal of Chemical Education, 85 (1), 130-132, 2008.

Xavier, A.M.R.B., Tavares, A.P.M., Ferreira R., Amado F., (2007) Trametes versicolor growth and laccase induction with by-products of pulp and paper industry. Electronic Journal of Biotechnology [online]. 15 Jully 2007, vol. 10, no. 3

Gamelas, J.A.F., Pontes, A.S.N., Evtuguin, D.V., Xavier, A.M.R.B., Esculcas A.P.  (2007) New polyoxometalate-laccase integrated system for kraft pulp delignification, Biochemistry Engineering Journal, 33 (2007) 141-147.

A.P.M. Tavares, M.A.Z. Coelho, M.S.M. Agapito, J.A.P. Coutinho and A.B.M.R. Xavier, Optimization and modelling of laccase production by Trametes versicolor in a Bioreactor using Statistical Experimental Design 2006 Applied Biochemistry and Biotechnology, 134, 233-248.


Gamelas, J.A.F., Tavares, A.P.M., Evtuguin, D.V., Xavier, A.M.R.B. “Oxygen bleaching of kraft pulp with polyoxometalates and laccase applying multi-stage process: comparative study”. Journal of Molecular Catalysis B: Enzymatic, 2005, 33, 57-64.

Tavares, A.P.M., Coelho, M.A.Z., Coutinho, J.A.P., Xavier, A.M.R.B. Laccase improvement in submerged cultivation: induced production and kinetic modeling. Journal of Chemical Technology and Biotechnology, 2005, 80, 669-676.

Tavares, A.P.M., Agapito, M.S.M., Coelho, M.A.Z., Lopes da Silva, J. A., Barros-Timmons A., Coutinho, J.A.P., Xavier, A.M.R.B. Selection and optimization of culture medium for exopolysaccharide production by Coriolus (Trametes) versicolor, World Journal of Microbiology and Biotechnology, 2005, 21, 1499-1507.

A.P.M. Tavares, M.A.Z. Coelho, J.A.P. Coutinho, A.M.R.B. Xavier. Laccase improvement in submerged cultivation: induced production and kinetic modelling. Journal of Chemical Technology and Biotechnology, 80 688-696(2005).

J.A.F. Gamelas, A.P.M. Tavares, D.V. Evtuguin, A.M.B. Xavier. Oxygen bleaching of kraft pulp with polyoxometalates and laccase applying multi-stage process: comparative study. Journal of Molecular Catalysis B: Enzymatic, 33 57-64 (2005).

P.F.F. Amaral, A.P.M. Tavares, A.M.R.B. Xavier, M.C. Cammarota, J.A.P. Coutinho, M.A.Z. Coelho. Dye decolorization performed by Trametes versicolor from textile wastewater. Environmental Technology, 25 1313-1320 (2004).

A.P.M. Tavares, J.A.F. Gamelas, A. Gaspar, D.V. Evtuguin, A.M.R.B. Xavier. A novel approach for the oxidative catalysis employing polyoxometalate-laccase system: application to the oxygen bleaching of kraft pulp. Catalysis Communications, 5 485-489 (2004).

Castro, IRP, Evtuguin, DV, Xavier, AMRB, “Degradation of biphenyl lignin model compounds by laccase of Trametes versicolor in presence of 1-hydroxybenzotriazole and heteropolyanion [SiW11VO40]5-”, Journal of Molecular Catalysis B: Enzymatic, 2003, 22, 13-20.

Coutinho, J.A.P., Xavier, A.M.R.B (2000). A model for the micro/ultrafiltration cell deactivation in cell-recycle reactors. J. Chem. Tech & Biotech. 75: 315-319.

Gonçalves, L.M.D., A. Ramos, J.S. Almeida, A.M.R.B. Xavier, M.J.T. Carrondo (1997). Elucidation on the mechanism of lactic acid inhibition on growth and lactic acid production in batch cultures of Lactobacillus rhamnosus. Appl. Microbiol. Biotechnol.48: 345-350.

Xavier, Ana M.R.B., Crespo, J.P.S.G., Carrondo, M.J.T.(1997). Effect of cell purge on the stability of microfiltration cell recycle fermentation systems. J. Chem.Tech.Biotechnol.(1997). 69, 203-208.

A.M.R.B. Xavier, L.M.D.Gonçalves, J.L.Moreira and M.J. Carrondo,(1995) Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor. Biotechnol.Bioeng. 45: 320-327.

L.M.D.Gonçalves, M.T.O. Barreto, A.M.R.B. Xavier, M.J. Carrondo, and J. Klein,(1992) Inert supports for lactic acid fermentation - a technological assessment. Appl. Microbiol.and Biotechnol.38: 305-311.

J.P.Crespo, A.M.R.B.Xavier, M.T.O.Barreto, L.M.D.Gonçalves, J.S.Almeida, M.J.T.Carrondo,(1992) Tangencial flow filtration for continuos cell recycle culture of acidogenic bacteria. Chem. Eng Science 47/1, 205-214.

M.T.O.Barreto, E.P.Melo, J.S.Almeida, A.M.R.B.Xavier, M.T.J.Carrondo,(1991) A kinetic method for calculating the viability of lactic starter cultures. Appl Microbiol and Biotechnol 34, 648-652.

Gonçalves, L.M.D., Xavier, A.M.R.B., Almeida, J.S., Carrondo, M.J.T.,(1991) Concomitant substrate and product inhibition kinectics in lactic acid production. Enz and Microbial Tecnology 13, 314-319.
 

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