Methodology
- Methodology in solid-state NMR and Magic-Angle Spinning Dynamic Nuclear Polarization (MAS-DNP) techniques
- Developing of protocols for MAS-DNP
- Dipolar recoupling techniques by symmetry-based pulse sequences
- Other characterization techniques : Neutron scattering and refletometry, QCMd, AFM, ellipsometry.
- Sample preparation of lipidic samples: a)Langmuir-Blodgett film formation and liposome formation techniques
Materials
- Characterization of materials for gas retention (CO2, SF6) by MAS-DNP solid-state NMR
- NMR and MAS-DNP characterization of porous materials for application in catalysis
Biology
- Protein-Ligand interactions
- Inrteraction site structure in protein-ligand binding
- MAS-DNP and NMR characterization of biological systems
- Structural study of the interaction interfaces of clathrin adaptor proteins involved in clathrin-mediated endocytosis
- Endocytosis and its implications in degenerative diseases
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I obtained my BSc in Chemistry at the Universidad de Sevilla in 2001. By October 2003, I started a PhD programme funded by an EPSRC grant in the subject of Solid-State NMR (ssNMR) in the well-known group of Prof. Malcolm H. Levitt at the University of Southampton (UK). During my PhD, I acquired a solid theoretical and experimental knowledge of the NMR technique. As main outcome, I developed new methodology to improve homonuclear recoupling pulse sequences for the measurement of atomic distance, and its application to protein studies. In November 2007, I joined the group of Prof. Ulrich Günther at University of Birmingham, where I entered in contact for the first time with the Dynamic Nuclear Polarization (DNP) technique, which is revolutionizing NMR, improving by far the sensitivity limits of the technique with an important impact in areas like surface chemistry. These works were performed in close collaboration with the industrial partner Oxford Instruments Biotools, using the HypeSense equipment. I worked in new fast NMR methodology for its application in dissolution-DNP, the study of the polarization transfer mechanisms in DNP and the development of hardware for dissolution-DNP. By the end of 2010, I joined the Biomolecular NMR Laboratory of Prof. Miquel Pons at the IRB-Barcelona and the Universitat de Barcelona, after obtaining a “Beatriu de Pinos” and a “Juan de la Cierva” grants. The main research lines carried out were the characterization of paramagnetic supramolecular oligomers formed by trityl radicals, design and testing of new polarizing agents for DNP, the hyperpolarization of quadrupolar nuclei, like 14N, and the study of protein-ligand interactions by DNP-NMR. In 2015, I earned a Labex ARCANE Postdoctoral Fellowship to work in the laboratory of Dr. Gaël de Paëpe and Dr. Sabine Hediger at the CEA-Grenoble and Université Grenoble-Alpes in Grenoble (France). The main research line was the study of carbohydrate-protein interaction by MagicAngle-Spinning DNP (MAS-DNP) solid-state NMR in Lectins and application of MAS-DNP to the study of biomolecular problems. Additional projects involve the design and testing of new polarizing agents for MAS-DNP and its application to mesoporous materials, the mechanistic study of the polarization transfer process between electrons and nuclei, and the investigation of the influence of O2 in MAS-DNP experiments. Since January 2019, I have joined the CICECO institute of the Universidade de Aveiro to work in study of materials for the trapping of CO2 by ssNMR and MAS-DNP in collaboration with Dr. Mariana Sardo and Dr. Luis Mafra. Additionally, I will develop my own research line in biomolecular MAS-DNP with a project to selectively enlightening of the interaction interfaces of clathrin adaptor proteins involved in clathrin-mediated endocytosis.
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Mariana Sardo, Luis Mafra, João Rocha, Paula Santos, Ricardo Vieira, Baljeet Singh, Carlos Bornes, João Pereira,Rita Fonseca, Daniel Pereira.
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Selective High-resolution DNP-enhanced NMR of Biomolecular Binding SitesIldefonso Marin-Montesinos, David Goyard, Emilie Gillon, Olivier Renaudet, Anne Imberty,Sabine Hediger and Gaël De Paëpe 2019, Chemical Science, 10, 3366
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Detecting acetylated aminoacids in blood serum using hyperpolarized C-13-H-1-2D-NMRKatsikis, S; Marin-Montesinos, I; Ludwig, C; Gunther, UL 2019, JOURNAL OF MAGNETIC RESONANCE, 305, 175-179.
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Computationally Assisted Design of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR: The AsymPol FamilyF. Mentink-Vigier*, I. Marín-Montesinos*, A. P. Jagtap, J. van Tol, S. Hediger, D. Lee, S. Th. Sigurdsson, and G. De Paëpe 2018, Journal of the American Chemical Society, 140, 11013 -11019.
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NMR signal enhancement of more than 50 000 times in fast dissolution dynamic nuclear polarizationLuiz F. Pinto, Ildefonso Marin-Montesinos, Vega Lloveras, José Luís. Muñoz-Gómez, Miquel Pons, Jaume Veciana, José Vidal-Gancedo 2017, Chemical Communications, 53, 3757
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Paramagnetic spherical nanoparticles by the self- assembly of persistent trityl radicalsI. Marin-Montesinos, J. C. Paniagua, Alejandro Peman, M. Vilaseca, F. Luis, S. Van Doorslaer and M. Pons 2016, Physical Chemistry Chemical Physics, 18, 3151-3158
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Improved stability and spectral quality in ex situ Dissolution-DNP using and improved transfer deviceS. Katsikis, I. Marín-Montesinos, M. Pons, C. Ludwig and U. Günther 2015, Applied Magnetic Resonance, 46, 723
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Self-assembled trityl radical capsules. Implications for Dynamic Nuclear PolarizationI. Marín-Montesinos, J.C. Paniagua, M. Vilaseca, Ainhoa Urtizberea, Fernando Luís, M. Feliz, Feng Lin, S. Van Doorslaer and M. Pons 2015, Physical Chemistry Chemical Physics, 17, 5785
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Novel PTM-TEMPO biradical for Fast Dissolution Dynamic Nuclear PolarizationJ.L. Muñoz-Gómez, I. Marín-Montesinos, V. Lloveras, M. Pons, J. Vidal-Gancedo and J. Veciana 2014, Organic Letters, 16, 5402
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Long-lived states in an intrinsically disordered protein domainL. Fernandes, C. Guerniou, I. Marín-Montesinos, M. Pons, F. Kateb and P.R. Vasos 2013, Magnetic Resonance in Chemistry, 51, 729
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Optimizing the Polarization Matrix for ex Situ Dynamic Nuclear Polarization C. Ludwig*, I. Marin-Montesinos*, M. Saunders, and U. Günther 2010, Journal of the American Chemical Society, 132, 2508
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Quantum Rotor Induced HyperpolarizationM. Saunders, C. Ludwig, I. Marin-Montesinos and U. Günther, 2010, Proceedings of the National Academy of Sciences , 107, 10799
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Application of ex situ dynamic nuclear polarization in studying small moleculesntesinos, M. G. Saunders, A.H. Emwas, Z. Pikramenou, S.P. Hammond, U.L. Günther 2010, Physical Chemistry Chemical Physics , 12, 5868
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Light Penetration and Photoisomerization in Rhodopsin studied by Numerical Simulations and Double - Quantum Solid - State NMRM. Concistrè, A. Gansmüller, N. McLean, O. G. Johannessen, I. Marín Montesinos, P. H. M. Bovee- Geurts, R. C. D. Brown, W. J. DeGrip and M. H. Levitt, 2009, Journal of the American Chemical Society, 131, 6133
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Towards an interpretation of 13C chemical shifts in bathorhodopsin, a functional intermediate of a G-protein coupled receptorA. Gansmüller, M. Concistrè, N. McLean, O. G. Johannessen, I. Marín Montesinos, P. H. M. Bovee- Geurts, P. Verdegem, J. Lugtenburg, R. C. D. Brown, W. J. DeGrip and M. H. Levitt 2009, Biochimica et Biophysica Acta, 1788, 1350
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Double-Quantum 13C Nuclear Magnetic Resonance of Bathorhodopsin, the First Photointermediate in Mammalian VisionM. Concistrè, A. Gansmüller, N. McLean, O. G. Johannessen, I. Marín Montesinos, P. H. M. Bovee- Geurts, P. Verdegem, J. Lugtenburg, R. C. D. Brown, W. J. DeGrip and M. H. Levitt 2008, Journal of the American Chemical Society, 130, 10490
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