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Equipa
Mara Guadalupe Freire Martins
Investigador CoordenadorDescrição
With the emergence of antibiotic-resistant pathogens the development of antigen-specific antibodies for use in passive immunotherapy is, nowadays, a major concern in human society. Despite the most focused mammal antibodies, antibodies obtained from egg yolk of immunized hens, immunoglobulin Y (IgY), are an alternative option that can be obtained in higher titres by non-stressful and non-invasive methods. This large amount of available antibodies opens the door for a new kind of cheaper biopharmaceuticals. However, the production cost of high-quality IgY for large-scale applications remains higher than other drug therapies due to the lack of an efficient purification method. The search of new purification platforms is thus a vital demand to which liquid-liquid extraction using aqueous biphasic systems (ABS) could be the answer. Besides the conventional polymer-based systems, highly viscous and with a limited polarity/affinity range, a recent type of ABS composed of ionic liquids (ILs) may be employed. ILs are usually classified as “green solvents” due to their negligible vapour pressure. Yet, the major advantage of IL-based ABS relies on the possibility of tailoring their phases’ polarities aiming at extracting a target biomolecule. A proper manipulation of the system constituents and respective composition allows the pre-concentration, complete extraction, or purification of the most diverse biomolecules.
This research project addresses the development of a new technique for the extraction and purification of IgY from egg yolk using IL-based ABS. The proposed plan contemplates the optimization of purification systems at the laboratory scale and their use in countercurrent chromatography to achieve a simple, cost-effective and scalable process. The success of this project and its scalability to an industrial level certainly will allow the production of cheaper antibodies with a long-term impact in human healthcare."
Coordenador
Coordenação
Universidade de Aveiro (UA)
Participantes
Não
Resultados
Synthesis and characterization of analogues of glycine-betaine ionic liquids and their use in the formation of aqueous biphasic systems
Pereira, MM; Pedro, SN; Gomes, J; Sintra, TE; Ventura, SPM; Coutinho, JAP; Freire, MG; Mohamadou, ACan cholinium chloride form eutectic solvents with organic chloride-based salts?
Abranches, DO; Silva, LP; Martins, MAR; Fernandez, L; Pinho, SP; Coutinho, JAPOdd-Even Effect in the Formation and Extraction Performance of Ionic-Liquid-Based Aqueous Biphasic Systems
Belchior, DCV; Almeida, MR; Sintra, TE; Ventura, SPM; Duarte, IF; Freire, MGSolvatochromism as a new tool to distinguish structurally similar compounds
Madeira, PP; Loureiro, JA; Freire, MG; Coutinho, JAPBinary Mixtures of Ionic Liquids in Aqueous Solution: Towards an Understanding of Their Salting-In/Salting-Out Phenomena
Neves, CMSS; Dinis, TBV; Carvalho, PJ; Schroder, B; Santos, LMNBF; Freire, MG; Coutinho, JAPPolyvinylidene fluoride-Hyaluronic acid wound dressing comprised of ionic liquids for controlled drug delivery and dual therapeutic behavior
Abednejad, A; Ghaee, A; Morais, ES; Sharma, M; Neves, BM; Freire, MG; Nourmohammadi, J; Mehrizi, AAIonic Liquids in Bioseparation Processes
Belchior, DCV; Duarte, IF; Freire, MGStimuli responsive ion gels based on polysaccharides and other polymers prepared using ionic liquids and deep eutectic solvents
Prasad, K; Mondal, D; Sharma, M; Freire, MG; Mukesh, C; Bhatt, JCloud Point Extraction of Chlorophylls from Spinach Leaves Using Aqueous Solutions of Nonionic Surfactants
Leite, AC; Ferreira, AM; Morais, ES; Khan, I; Freire, MG; Coutinho, JAPMechanisms ruling the partition of solutes in ionic-liquid-based aqueous biphasic systems - the multiple effects of ionic liquids
Passos, H; Dinis, TBV; Capela, EV; Quental, MV; Gomes, J; Resende, J; Madeira, PP; Freire, MG; Coutinho, JAPEconomic evaluation of the primary recovery of tetracycline with traditional and novel aqueous two-phase systems
Torres-Acosta, MA; Pereira, JFB; Freire, MG; Aguilar-Yanez, JM; Coutinho, JAP; Titchener-Hooker, NJ; Rito-Palomares, MHydrogen bond basicity of ionic liquids and molar entropy of hydration of salts as major descriptors in the formation of aqueous biphasic systems
Passos, H; Dinis, TBV; Claudio, AFM; Freire, MG; Coutinho, JAPOdd-even effect on the formation of aqueous biphasic systems formed by 1-alkyl-3-methylimidazolium chloride ionic liquids and salts
Belchior, DCV; Sintra, TE; Carvalho, PJ; Soromenho, MRC; Esperanca, JMSS; Ventura, SPM; Rogers, RD; Coutinho, JAP; Freire, MGAn integrated process for enzymatic catalysis allowing product recovery and enzyme reuse by applying thermoreversible aqueous biphasic systems
Ferreira, AM; Passos, H; Okafuji, A; Tavares, APM; Ohno, H; Freire, MG; Coutinho, JAPDeep Eutectic Solvent Aqueous Solutions as Efficient Media for the Solubilization of Hardwood Xylans
Morais, ES; Mendonca, PV; Coelho, JFJ; Freire, MG; Freire, CSR; Coutinho, JP; Silvestre, AJDValorization of olive tree leaves: Extraction of oleanolic acid using aqueous solutions of surface-active ionic liquids
Claudio, AFM; Cognigni, A; de Faria, ELP; Silvestre, AJD; Zirbs, R; Freire, MG; Bica, KEnhanced separation performance of aqueous biphasic systems formed by carbohydrates and tetraalkylphosphonium- or tetraalkylammonium-based ionic liquids
Quental, MV; Pereira, MM; Ferreira, AM; Pedro, SN; Shahriari, S; Mohamadou, A; Coutinho, JAP; Freire, MGPioneering Use of Ionic Liquid-Based Aqueous Biphasic Systems as Membrane-Free Batteries
Navalpotro, P; Neves, CMSS; Palma, J; Freire, MG; Coutinho, JAP; Marcilla, RSwitchable (pH-driven) aqueous biphasic systems formed by ionic liquids as integrated production-separation platforms
Ferreira, AM; Claudio, AFM; Valega, M; Domingues, FMJ; Silvestre, AJD; Rogers, RD; Coutinho, JAP; Freire, MGSolvatochromic parameters of deep eutectic solvents formed by ammonium-based salts and carboxylic acids
Teles, ARR; Capela, EV; Carmo, RS; Coutinho, JAP; Silvestre, AJD; Freire, MGAlternative probe for the determination of the hydrogen-bond acidity of ionic liquids and their aqueous solutions
Madeira, PP; Passos, H; Gomes, J; Coutinho, JAP; Freire, MGDesigning the thermal behaviour of aqueous biphasic systems composed of ammonium-based zwitterions
Ferreira, AM; Passos, H; Okafuji, A; Freire, MG; Coutinho, JAP; Ohno, HImproved monitoring of aqueous samples by the preconcentration of active pharmaceutical ingredients using ionic-liquid-based systems
Almeida, HFD; Freire, MG; Marrucho, IMTemperature dependency of aqueous biphasic systems: an alternative approach for exploring the differences between Coulombic-dominated salts and ionic liquids
Silva, FAE; Pereira, JFB; Kurnia, KA; Ventura, SPM; Silva, AMS; Rogers, RD; Coutinho, JAP; Freire, MGIonic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends
Ventura, SPM; Silva, FAE; Quental, MV; Mondal, D; Freire, MG; Coutinho, JAPEffective separation of aromatic and aliphatic amino acid mixtures using ionic-liquid-based aqueous biphasic systems
Capela, EV; Quental, MV; Domingues, P; Coutinho, JAP; Freire, MGA Triple Salting-Out Effect is Required for the Formation of Ionic-Liquid-Based Aqueous Multiphase Systems
Passos, H; Costa, SH; Fernandes, AM; Freire, MG; Rogers, RD; Coutinho, JAPLong-term protein packaging in cholinium-based ionic liquids: improved catalytic activity and enhanced stability of cytochrome c against multiple stresses
Bisht, M; Mondal, D; Pereira, MM; Freire, MG; Venkatesu, P; Coutinho, JAPToward an Understanding of the Mechanisms behind the Formation of Liquid-liquid Systems formed by Two Ionic Liquids
Neves, CMSS; Silva, AMS; Fernandes, AM; Coutinho, JAP; Freire, MGDensities, viscosities and derived thermophysical properties of water-saturated imidazolium-based ionic liquids
Martins, MAR; Neves, CMSS; Kurnia, KA; Carvalho, PJ; Rocha, MAA; Santos, LMNBF; Pinho, SP; Freire, MGImproved extraction of fluoroquinolones with recyclable ionic-liquid-based aqueous biphasic systems
Almeida, HFD; Freire, MG; Marrucho, IMEnhanced tunability afforded by aqueous biphasic systems formed by fluorinated ionic liquids and carbohydrates
Ferreira, AM; Esteves, PDO; Boal-Palheiros, I; Pereiro, AB; Rebelo, LPN; Freire, MGInfluence of Nanosegregation on the Surface Tension of Fluorinated Ionic Liquids
Luis, A; Shimizu, K; Araujo, JMM; Carvalho, PJ; Lopes-da-Silva, JA; Lopes, JNC; Rebelo, LPN; Coutinho, JAP; Freire, MG; Pereiro, ABThe effect of n vs. iso isomerization on the thermophysical properties of aromatic and non-aromatic ionic liquids
Rodrigues, ASMC; Almeida, HFD; Freire, MG; Lopes-da-Silva, JA; Coutinho, JAP; Santos, LMNBFSolid-liquid equilibria of binary mixtures of fluorinated ionic liquids
Teles, ARR; Correia, H; Maximo, GJ; Rebelo, LPN; Freire, MG; Pereiro, AB; Coutinhoa, JAPFluorination effects on the thermodynamic, thermophysical and surface properties of ionic liquids
Vieira, NSM; Luis, A; Reis, PM; Carvalho, PJ; Lopes-da-Silva, JA; Esperanca, JMSS; Araujo, JMM; Rebelo, LPN; Freire, MG; Pereiro, ABThermoreversible (Ionic-Liquid-Based) Aqueous Biphasic Systems
Passos, H; Luis, A; Coutinho, JAP; Freire, MGSuitability of bio-based ionic liquids for the extraction and purification of IgG antibodies
Mondal, D; Sharma, M; Quental, MV; Tavares, APM; Prasad, K; Freire, MGA critical assessment of the mechanisms governing the formation of aqueous biphasic systems composed of protic ionic liquids and polyethylene glycol
Claudio, AFM; Pereira, JFB; McCrary, PD; Freire, MG; Coutinho, JAP; Rogers, RDAqueous biphasic systems composed of ionic liquids and polypropylene glycol: insights into their liquid-liquid demixing mechanisms
Neves, CMSS; Shahriari, S; Lemus, J; Pereira, JFB; Freire, MG; Coutinho, JAPImproving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems
Ferreira, AM; Faustino, VFM; Mondal, D; Coutinho, JAP; Freire, MGIonic liquids in chromatographic and electrophoretic techniques: toward additional improvements in the separation of natural compounds
Soares, B; Passos, H; Freire, CSR; Coutinho, JAP; Silvestre, AJD; Freire, MGSingle-step purification of ovalbumin from egg white using aqueous biphasic systems
Pereira, MM; Cruz, RAP; Almeida, MR; Lima, AS; Coutinho, JAP; Freire, MGSurface tensions of ionic liquids: Non-regular trend along the number of cyano groups
Almeida, HFD; Carvalho, PJ; Kurnia, KA; Lopes-da-Silva, JA; Coutinho, JAP; Freire, MGSolubility and solvation of monosaccharides in ionic liquids
Teles, ARR; Dinis, TBV; Capela, EV; Santos, LMNBF; Pinho, SP; Freire, MG; Coutinho, JAPThermophysical Properties of Two Ammonium-Based Protic Ionic Liquids
Bhattacharjee, A; Coutinho, JAP; Freire, MG; Carvalho, PJAqueous Biphasic Systems Composed of Ionic Liquids and Acetate-Based Salts: Phase Diagrams, Densities, and Viscosities
Quental, MV; Passos, H; Kurnia, KA; Coutinho, JAP; Freire, MGControlling the Formation of Ionic-Liquid-based Aqueous Biphasic Systems by Changing the Hydrogen-Bonding Ability of Polyethylene Glycol End Groups
Pereira, JFB; Kurnia, KA; Freire, MG; Coutinho, JAP; Rogers, RDComprehensive study on the impact of the cation alkyl side chain length on the solubility of water in ionic liquids
Kurnia, KA; Neves, CMSS; Freire, MG; Santos, LMNBF; Coutinho, JAPMutual solubilities between water and non-aromatic sulfonium-, ammonium- and phosphonium-hydrophobic ionic liquids
Kurnia, KA; Quental, MV; Santos, LMNBF; Freire, MG; Coutinho, JAPExtraction and stability of bovine serum albumin (BSA) using cholinium-based Good's buffers ionic liquids
Taha, M; Quental, MV; Correia, I; Freire, MG; Coutinho, JAPThermophysical properties of phosphonium-based ionic liquids
Bhattacharjee, A; Lopes-da-Silva, JA; Freire, MG; Coutinho, JAP; Carvalho, PJThe magic of aqueous solutions of ionic liquids: ionic liquids as a powerful class of catanionic hydrotropes
Claudio, AFM; Neves, MC; Shimizu, K; Lopes, JNC; Freire, MG; Coutinho, JAPHydrogen-bond acidity of ionic liquids: an extended scale
Kurnia, KA; Lima, F; Claudio, AFM; Coutinho, JAP; Freire, MGEffect of the Methylation and N-H Acidic Group on the Physicochemical Properties of Imidazolium-Based Ionic Liquids
Rodrigues, ASMC; Rocha, MAA; Almeida, HFD; Neves, CMSS; Lopes-da-Silva, JA; Freire, MG; Coutinho, JAP; Santos, LMNBFGood's buffers as novel phase-forming components of ionic-liquid-based aqueous biphasic systems
Luis, A; Dinis, TBV; Passos, H; Taha, M; Freire, MGOne-step extraction and concentration of estrogens for an adequate monitoring of wastewater using ionic-liquid-based aqueous biphasic systems
Dinis, TBV; Passos, H; Lima, DLD; Esteves, VI; Coutinho, JAP; Freire, MGNovel Biocompatible and Self-buffering Ionic Liquids for Biopharmaceutical Applications
Taha, M; Almeida, MR; Silva, FAE; Domingues, P; Ventura, SPM; Coutinho, JAP; Freire, MGContact angles and wettability of ionic liquids on polar and non-polar surfaces
Pereira, MM; Kurnia, KA; Sousa, FL; Silva, NJO; Lopes-da-Silva, JA; Coutinhoa, JAP; Freire, MGEffect of salts on the solubility of ionic liquids in water: experimental and electrolyte Perturbed-Chain Statistical Associating Fluid Theory
Neves, CMSS; Held, C; Mohammad, S; Schleinitz, M; Coutinho, JAP; Freire, MGThermophysical properties of sulfonium- and ammonium-based ionic liquids
Bhattacharjee, A; Luis, A; Santos, JH; Lopes-da-Silva, JA; Freire, MG; Carvalho, PJ; Coutinho, JAPAqueous two-phase systems formed by biocompatible and biodegradable polysaccharides and acetonitrile
Cardoso, GDB; Souz, IN; Pereira, MM; Freire, MG; Soares, CMF; Lima, ASThe effect of the cation alkyl chain branching on mutual solubilities with water and toxicities
Kurnia, KA; Sintra, TE; Neves, CMSS; Shimizu, K; Lopes, JNC; Goncalves, F; Ventura, SPM; Freire, MG; Santos, LMNBF; Coutinho, JAPThe impact of ionic liquid fluorinated moieties on their thermophysical properties and aqueous phase behaviour
Neves, CMSS; Kurnia, KA; Shimizu, K; Marrucho, IM; Rebelo, LPN; Coutinho, JAP; Freire, MG; Lopes, JNCIonic liquid solutions as extractive solvents for value-added compounds from biomass
Passos, H; Freire, MG; Coutinho, JAPGood's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
Taha, M; Silva, FAE; Quental, MV; Ventura, SPM; Freire, MG; Coutinho, JAPFinanciadores
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