Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion


The correct choice of the specific lignocellulosic biomass pretreatment allows obtaining high biomass conversions for biorefinery implementations and cellulosic bioethanol production from renewable resources. Cynara cardunculus (cardoon) pretreated by steam explosion (SE) was involved in second-generation bioethanol production using separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) processes. Steam explosion pretreatment led to partial solubilisation of hemicelluloses and increased the accessibility of residual polysaccharides towards enzymatic hydrolysis revealing 64% of sugars yield against 11% from untreated plant material. Alkaline extraction after SE pretreatment of cardoon (CSEOH) promoted partial removal of degraded lignin, tannins, extractives and hemicelluloses thus allowing to double glucose concentration upon saccharification step. Bioethanol fermentation in SSF mode was faster than SHF process providing the best results: ethanol concentration 18.7 g L-1, fermentation efficiency of 66.6% and a yield of 26.6 g ethanol/100 g CSEOH or 10.1 g ethanol/100 g untreated cardoon. (C) 2015 Elsevier Ltd. All rights reserved.



subject category

Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels


Fernandes, MC; Ferro, MD; Paulino, AFC; Mendes, JAS; Gravitis, J; Evtuguin, DV; Xavier, AMRB

our authors


This research was co-financed by INALENTEJO 2007.2013/QREN, within the project BioEcos-Valorizacao Integrada de Biomassa and FEDER - Portugal funds through COMPETE (Programa Operacional Factores de Competitividade) and within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013), financed by Portuguese national funds through the FCT/MEC.; The steam explosion treatment of biomass has been supported by the Latvian National Research Programme

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