abstract
This work proposes the application of flexible alcoholic aqueous two-phase systems to manipulate the partition of caffeine. An optimization study was performed the caffeine partitioning towards top phase (60.0 wt% 2-propanol + 20.0 wt% K3PO4 at 303 K - K-caf = 3.4 and R-T = 89.1%) and bottom phase (60.0 wt% methanol + 17.5 wt% K2HPO4/KH2PO4 at 278 K - K-caf = 0.06 and R-B = 81.1%). These processes were evaluated by applying them to real biomass as guarana seeds (purification factor (PF) = 6.59-fold) and coffee beans (PF = 3.24-fold) for the 2-propanol and K2HPO4/KH2PO4 system.
keywords
LIQUID-LIQUID-EXTRACTION; IONIC LIQUIDS; GREEN TEA; LIPASE PURIFICATION; PHASE-DIAGRAMS; BIPHASIC SYSTEMS; WATER; RECOVERY; SAMPLES; ACETONITRILE
subject category
Chemistry; Engineering
authors
Santos, SB; Reis, IAO; Silva, CPC; Campos, AF; Ventura, SPM; Soares, CMF; Lima, AS
our authors
Sónia Patrícia Marques Ventura
Assistant Professoracknowledgements
The authors are grateful for the financial support from FAPITEC, for the scholarship of I.A.O. Reis and S.B. Santos, and CAPES for the scholarship of S.B. Santos. This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013), financed by national funds through the FCT/MEC and when applicable co-financed by FEDER under the PT2020 Partnership Agreement. The authors also thank Fundacao para a Ciencia e a Tecnologia (FCT) for the post-doctoral grant SFRH/BPD/79263/2011 of S.P.M. Ventura.