Cell encapsulation in liquified compartments: Protocol optimization and challenges


Cell encapsulation is a widely used technique in the field of Tissue Engineering and Regenerative Medicine (TERM). However, for the particular case of liquefied compartmentalised systems, only a limited number of studies have been reported in the literature. We have been exploring a unique cell encapsulation system composed by liquefied and multilayered capsules. This system transfigured the concept of 3D scaffolds for TERM, and was already successfully applied for bone and cartilage regeneration. Due to a number of appealing features, we envisage that it can be applied in many other fields, including in advanced therapies or as disease models for drug discovery. In this review, we intend to highlight the advantages of this new system, while discussing the methodology, and sharing the protocol optimization and results. The different liquefied systems for cell encapsulation reported in the literature will be also discussed, considering the different encapsulation matrixes as core templates, the types of membranes, and the core liquefaction treatments.



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Science & Technology - Other Topics


Correia, CR; Ghasemzadeh-Hasankolaei, M; Mano, JF

our authors


The authors acknowledge the funding received from the European Research Council (ERC) for project ATLAS (ERC-2014-ADG-669858), and the Portuguese Foundation for Science and Technology (FTC) for the project CIRCUS (PTDC/BTM-MAT/31064/2017). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, FCT Ref. UID/CTM/50011/2019, financed by national funds through the FCT/MCTES. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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