One-Minute Synthesis of Size-Controlled Fucoidan-Gold Nanosystems: Antitumoral Activity and Dark Field Imaging
authors Pinto, RJB; Bispo, D; Vilela, C; Botas, AMP; Ferreira, RAS; Menezes, AC; Campos, F; Oliveira, H; Abreu, MH; Santos, SAO; Freire, CSR
nationality International
author keywords gold nanoparticles; fucoidan; microwave irradiation; antitumoral activity; darkfield imaging
abstract Gold nanoparticles (AuNPs) are one of the most studied nanosystems with great potential for biomedical applications, including cancer therapy. Although some gold-based systems have been described, the use of green and faster methods that allow the control of their properties is of prime importance. Thus, the present study reports a one-minute microwave-assisted synthesis of fucoidan-coated AuNPs with controllable size and high antitumoral activity. The NPs were synthesized using a fucoidan-enriched fraction extracted from Fucus vesiculosus, as the reducing and capping agent. The ensuing monodispersed and spherical NPs exhibit tiny diameters between 5.8 and 13.4 nm for concentrations of fucoidan between 0.5 and 0.05% (w/v), respectively, as excellent colloidal stability in distinct solutions and culture media. Furthermore, the NPs present antitumoral activity against three human tumor cell lines (MNT-1, HepG2, and MG-63), and flow cytometry in combination with dark-field imaging confirmed the cellular uptake of NPs by MG-63 cell line.
publisher MDPI
isbn 1996-1944
year published 2020
volume 13
issue 5
digital object identifier (doi) 10.3390/ma13051076
web of science category Materials Science, Multidisciplinary
subject category Materials Science
unique article identifier WOS:000524060200054
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journal impact factor 3.057
5 year journal impact factor 3.424
category normalized journal impact factor percentile 58.121
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