Magnetic Chitosan Bionanocomposite Films as a Versatile Platform for Biomedical Hyperthermia

abstract

Responsive magnetic nanomaterials offer significant advantages for innovative therapies, for instance, in cancer treatments that exploit on-demand delivery on alternating magnetic field (AMF) stimulus. In this work, biocompatible magnetic bionanocomposite films are fabricated from chitosan by film casting with incorporation of magnetite nanoparticles (MNPs) produced by facile one pot synthesis. The influence of synthesis conditions and MNP concentration on the films' heating efficiency and heat dissipation are evaluated through spatio-temporal mapping of the surface temperature changes by video-thermography. The cast films have a thickness below 100 mu m, and upon exposure to AMF (663 kHz, 12.8 kA m-1), induce exceptionally strong heating, reaching a maximum temperature increase of 82 degrees C within 270 s irradiation. Further, it is demonstrated that the films can serve as substrates that supply heat for multiple hyperthermia scenarios, including: i) non-contact automated heating of cell culture medium, ii) heating of gelatine-based hydrogels of different shapes, and iii) killing of cancerous melanoma cells. The films are versatile components for non-contact stimulus with translational potential in multiple biomedical applications. The strong heating efficiency of magnetic chitosan bionanocomposite films, with thickness below 100 mu m, is studied by video thermography under alternating magnetic field (AMF). High hyperthermia (MH) efficiency and precise temperature control using AMF are demonstrated by MH treatment of MNT-1 cells. The cell's viability drastically decreases below 10%, 48 h after a single AMF cycle of 10 min.image

keywords

NANOPARTICLES; MECHANISM; TISSUE

subject category

Engineering; Science & Technology - Other Topics; Materials Science

authors

Barra, A; Wychowaniec, JK; Winning, D; Cruz, MM; Ferreira, LP; Rodriguez, BJ; Oliveira, H; Ruiz-Hitzky, E; Nunes, C; Brougham, DF; Ferreira, P

Groups

G2 - Photonic, Electronic and Magnetic Materials
G4 - Renewable Materials and Circular Economy

Projects

Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

Embalagens em biocompósitos para preservação ativa de alimentos (BIOFOODPACK)

acknowledgements

This work was developed within the scope of the projects CICECO - Aveiro Institute of Materials UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, CESAM -UIDP/50017/2020, and UIDB/50017/2020 and LA/P/0094/2020 and M-ERA-NET2/0021/2016 - BIOFOODPACK - Biocomposite Packaging for Active Preservation of Food. A.B. is thankful to FCT for grant SFRH/BD/148856/2019. C.N. is grateful to the Portuguese national funds (OE) through FCT IP in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of the Article 23 of the Decree-Law 57/2016 of August 29 changed by Law 57/2017 of July 19. J.K.W., D.W., and D.F.B. acknowledge support from the Science Foundation Ireland (16/IA/4584). E.R.-H. acknowledges financial support from the MCIN/AEI/10.13039/501100011033 (Spain, project PID2019-105479RB-I00). M.M.C. and L.P.F. acknowledge FCT for Centre grants UIDB/04046/2020 and UIDP/04046/2020 to BioISI. Part of the work in this article is based on the mobility exchange program of COST Action CA18132, supported by COST (European Cooperation in Science and Technology). FCT is also acknowledged for the research contract under Scientific Employment Stimulus to H.O. (CEECIND/04050/2017).Open access funding provided by IReL.