abstract
The fishing industry produces substantial by-products, such as heads, skins, bones, and scales, rich in collagen-a prevalent protein in these materials. However, further application of deep eutectic solvent-based extraction remains unexplored. In this study, we extracted collagen with urea: propanoic acid mixture (U:PA; 1:2) with a 2.2 % yield, followed by enzymatic hydrolysis with alcalase for 120 min. The resulting bioactive peptides demonstrated notable antioxidant activity (961 mu mol TE) and antihypertensive properties (39.3 % ACE inhibi-tion). Subsequently, we encapsulated 39.3 % of these hydrolysates in chitosan-TPP capsules, which released about 58 % of their content, primarily in the intestine, as mimicked in the in vitro model of the gastrointestinal tract. Although the digestion process did not significantly alter the size of the non-encapsulated collagen pep-tides, it did influence their health benefits. The promising results suggest that further research could optimize the use of collagen from fish by-products, potentially offering a sustainable source for health products.
keywords
ANTIOXIDANT; PEPTIDES; ENTRAPMENT; RELEASE; PROTEIN; ENCAPSULATION; HYDROGEL; SYSTEM; BEADS
subject category
Food Science & Technology
authors
Silva, I; Vaz, BMC; Sousa, S; Pintado, MM; Coscueta, ER; Ventura, SPM
our authors
Bárbara Maria Correia Vaz
PhD Student
Sónia Patrícia Marques Ventura
Assistant ProfessorProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was supported by National Funds from FCT-Fundacao para a Ciencia e a Tecnologia through project UIDB/50016/2020. Also, this work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MCTES (PIDDAC) .