Bio-based solvents as a sustainable alternative to develop a multiproduct biorefinery process from archaebacteria Halobacterium salinarum R1

abstract

This study delves into an innovative biorefinery approach to extract multiple high-value compounds from a single biomass source, Halobacterium salinarum R1, a resilient halophilic microorganism. By using bio-based solvents, namely an aqueous solution of gamma-valerolactone (GVL) and ethanol, a simple and efficient pipeline approach was developed, recovering unique pigments, including C-50 bacterioruberin, as well as two additional fractions consisting of protein and polysaccharides. The process is based on sustainable engineering and green chemistry principles, providing a viable alternative to replacing non-renewable solvents. The study addresses environmental concerns by employing bio-based solvents while presenting a cost-effective and sustainable solution. This approach contributes to developing a high-performance and sustainable alternative, promoting the development of a blue bioeconomy.

keywords

AQUEOUS-SOLUTIONS; GAMMA-VALEROLACTONE; IONIC LIQUIDS; EXTRACTION; MICROALGAE; GREEN; CAROTENOIDS; SOLUBILITY; PIGMENTS

subject category

Chemistry; Science & Technology - Other Topics

authors

Kholany, M; Macário, IPE; Veloso, T; Contieri, LS; Vaz, BMC; Pereira, JL; Nunes, C; Coutinho, JAP; Rostagno, MA; Ventura, SPM; Mesquita, LMD

Groups

G4 - Renewable Materials and Circular Economy

Projects

Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

PTNMR - Portuguese Nuclear Magnetic Resonance Network (REDE/1517/RMN/2005)

acknowledgements

This work was supported by "Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP" through the projects (2018/14582-5 and 2019/13496-0) and fellowships (L. M. de S. M.: 2020/08421-9, 2021/11022-1; LCS: 2020/03623-2, 2021/11023-8). This work was supported by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) (productivity grant 302610/2021-9). This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). The authors acknowledge FTC/MCTES for the financial support to CESAM, UIDB/50017/2020 + UIDP/50017/2020 + LA/P/0094/2020, financed by national funds. The authors are also grateful to the FCT for the doctoral grants of M. Kholany (SFRH/BD/138413/2018), I. P. E. Macario (SFRH/BD/123850/2016), and T. Veloso (SFRH/BD/147346/2019). The National NMR Network, funded within the framework of the National Program for Scientific Re-equipment, contract REDE/1517/RMN/2005 with funds from POCI 2010 (FEDER) and FCT, is also acknowledged. CN is grateful to Portuguese national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.