abstract
Cell culture-based technologies are widely utilized in various domains such as drug evaluation, toxicity assessment, vaccine and biopharmaceutical development, reproductive technology, and regenerative medicine. It has been demonstrated that pre -adsorption of extracellular matrix (ECM) proteins including collagen, laminin and fibronectin provide more degrees of support for cell adhesion. The purpose of cell imprinting is to imitate the natural topography of cell membranes by gels or polymers to create a reliable environment for the regulation of cell function. The results of recent studies show that cell imprinting is a tool to guide the behavior of cultured cells by controlling their adhesive interactions with surfaces. Therefore, in this review we aim to compare different cell cultures with the imprinting method and discuss different cell imprinting applications in regenerative medicine, personalized medicine, disease modeling, and cell therapy.
keywords
MESENCHYMAL STEM-CELLS; ARTICULAR CHONDROCYTES; EXTRACELLULAR-MATRIX; CANCER-CELLS; MICROFLUIDIC DEVICES; TISSUE ARCHITECTURE; DRUG DISCOVERY; CULTURE; THERAPY; CARTILAGE
subject category
Pharmacology & Pharmacy
authors
Hasannejad, F; Montazeri, L; Mano, JF; Bonakdar, S; Fazilat, A
our authors
João Mano
Full professoracknowledgements
We would like to thank Pasteur Institute and Motamed Cancer Institute of Iran for supporting this research. We would also like to thank our colleagues at Royan Institute and University of Aveiro who gave insight and knowledge that considerably aided the research.