abstract
Heart failure (HF) is a clinical entity included in cardiovascular diseases affecting millions of people worldwide, being a leading cause of hospitalization of older adults, and therefore imposing a substantial economic burden on healthcare systems. HF is characterized by dyspnea, fatigue, and edema associated with elevated blood levels of natriuretic peptides, such as N Terminal pro-B-type Natriuretic Peptide (NT-proBNP), for which there is a high demand for point of care testing (POCT) devices. Optical fiber (OF) biosensors offer a promising solution, capable of real-time detection, quantification, and monitoring of NT-proBNP concentrations in serum, saliva, or urine. In this study, immunosensors based on plasmonic uncladded OF tips were developed using OF with different core diameters (200 and 600 mu m). The tips were characterized to bulk refractive index (RI), anddetection tests were conducted with NT-proBNP concentrations varying from 0.01 to 100 ng/mL. The 200 mu m sensors showed an average total variation of 3.6 +/- 2.5 mRIU, an average sensitivity of 50.5 mRIU/ng
keywords
HEART-FAILURE; IMMUNOSENSOR; DIAGNOSIS
subject category
Chemistry; Science & Technology - Other Topics; Instruments & Instrumentation
authors
Assunçao, AS; Vidal, M; Martins, MJ; Girao, AV; Loyez, M; Caucheteur, C; Mesquita-Bastos, J; Costa, FM; Pereira, SO; Leitao, C
our authors
Ana Violeta Girão
Junior Researcher
Sónia Oliveira Pereira
Research FellowshipProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
This work was developed and financed within the scope of the project i3N, LA/P/0037/2020, UIDB/50025/2020 & UIDP/50025/2020, and the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). C. Leit & atilde;o was funded by FCT through the CEECIND/00154/2020 contract.