Antibiograms, multidrug resistance, and milk-related parameters of bacteria isolated from milk of dairy cattle in Phatthalung, Thailand

abstract

Background and Aim: Milk, a nutritious food, is widely consumed in human diets; however, contamination by microorganisms can negatively impact its quality and consumer health. Contamination by micro-organisms affects the quality of milk, which can affect the quality of the milk production chain. This study aimed to determine the changes in milk composition and antibiotic susceptibility related to bacteria isolated from dairy cow milk. Materials and Methods: Raw milk samples were collected from 72 dairy cows. All milk samples were subjected to the California Mastitis Test (CMT) for CMT score determination. We also investigated milk composition, bacterial culture (BC), and antibiotic susceptibility. Results: About 47.22% and 30.56% of dairy cattle were positive for CMT + BC and automatic somatic cell count (ASCC) + BC, respectively. Fecal appearance and animal age were found to be risk factors for ASCC + BC positivity in dairy cattle. Bacteria were found in approximately 76% of milk samples, with the most common isolated species being alpha -hemolytic Streptococcus spp., coagulase-negative Staphylococcus spp., and Escherichia coli . Of these, 70% are resistant to at least one antibiotic. Variation in the multidrug resistance pattern was high in Klebsiella spp. Conclusions: Fecal appearance and animal age are risk factors for ASCC + BC positivity in dairy cattle. This study identified antibiotic and multidrug resistance patterns, which require comprehensive studies and effective surveillance systems. Remarkably, the use of antibiotic therapy in dairy cattle should be monitored.

keywords

BOVINE SUBCLINICAL MASTITIS; SOMATIC-CELL COUNTS; BULK TANK MILK; ANTIMICROBIAL RESISTANCE; FECAL CONSISTENCY; PREVALENCE; COWS; CLEANLINESS; ENVIRONMENT; VIRULENCE

subject category

Agriculture; Veterinary Sciences

authors

Somrup, S; Mitsuwan, W; Bhumibhamon, T; Pereira, MD; Paul, AK; Nissapatorn, V; Saengsawang, P

Groups

G5 - Biomimetic, Biological and Living Materials

Projects

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

acknowledgements

The authors thank all staff and undergraduate students of the Faculty of Technology and Community Development, Thaksin University, and the Veterinary Research and Development Center (Upper Southern Region) , Department of Livestock Development, Ministry of Agriculture and Cooperatives, Thailand, who assisted with milk sample collection and laboratory processes. This study was supported by the Thaksin University Research Fund (Grant number DR25640001200-46) , Research and Development Institute, Thaksin University, Thailand. Thanks are due to project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 (Governo De Portugal, Instituto de Materiais de Aveiro - CICECO, Portugal) and LA/P/0006/2020, financed by National funds through the FCT/MEC (PIDDAC) .