abstract
In this work, a performance comparison between two Surface Plasmon Resonance (SPR) immunosensors for detecting ochratoxin A (OTA) in aqueous solutions is reported. More specifically, two SPR probes based on spoon-shaped waveguides and plastic optical fibers (POFs) were functionalized with the same anti-OTA receptor in order to assess and compare their biosensing performance. In both sensor configurations, the measurements pointed out a good linear relation among the variation in SPR wavelength values and the OTA concentration range from 0.2 ppt to 5 ppt, achieving binding sensitivities of about 1233 nm/ppb and 924 nm/ppb, and detection limits of 0.17 ppt and 0.18 ppt for the probe based on a spoon-shaped waveguide and POF, respectively. Each developed and tested polymer-based SPR biosensor presents an ultra-high sensitivity via a low-cost setup, representing an approach to realize portable analytical devices for on-site qualitative and quantitative OTA measurements in several application fields, like in coffee quality analyses.
keywords
OPTICAL-FIBER; SENSITIVE DETECTION; POLYPYRROLE FILM; BIOSENSOR; WINE; SPR
subject category
Computer Science; Engineering
authors
Marques, C; Silva, TD; Zeni, L; de Oliveira, JP; Arcadio, F; Cennamo, N
our authors
Carlos Marques
Assistant ProfessorProjects
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
We gratefully acknowledge the financial support from the financial support of CICECO (LA/P/0006/2020, UIDP/50011/2020, UIDB/50011/2020) funded by national funds through the FCT.