abstract
Wound care is a critical issue for healthcare systems, which translates into an increased demand for innovative wound dressings that can expedite the healing process while ensuring patient comfort and safety. In this context, the development of sustainable biopolymeric materials for wound care applications has gained considerable interest. In this study, functional biopolymeric films, composed of xylans, chitosan, and a deep eutectic solvent (DES), were prepared by solvent casting. The DES was simultaneously used as the solubilization medium for the biopolymers and as plasticizer and compatibilizer of xylans and chitosan. The film with the best composition (50:50 w/w ratio of xylan:chitosan) presented good homogeneity, thermal stability up to 150 degrees C, adequate mechanical properties (tensile strength up to 8.2 MPa and elongation at break up to 110 %), non-cytotoxicity towards human keratinocytes (HaCaT cell line, cell viability >= 80 %), UV-light protection (transmittance <= 38 %, 200-400 nm), and good antibacterial activity against a methicillin-resistant Staphylococcus aureus (MRSA) strain (>= 4-log CFU center dot mL-1 reduction, in vitro assay; 2-log CFU center dot mL-1 reduction, ex vivo assay). Finally, in vitro wound healing 2D scratch assays showed 76 % wound area closure after 30 h, and 17 % of relative cell viability in the simulated wound area after 72 h achieved with a 3D hydrogel model.
keywords
HYDROGEL; HEMICELLULOSE; ACID; ANTIBACTERIAL; DERIVATIVES; FABRICATION; COMPLEXES; DRESSINGS; CARTILAGE; PRODUCTS
subject category
Biochemistry & Molecular Biology; Chemistry; Polymer Science
authors
Silva, JM; Carvalho, JPF; Teixeira, MC; Facchinatto, WM; Braz, M; Almeida, A; Oliveira, H; Vilela, C; Branco, PC; Martins, J; Freire, MG; Freire, CSR; Silvestre, AJD
our authors
Armando Jorge Domingues Silvestre
Full professor
Carla Vilela
Assistant Professor
Carmen Sofia da Rocha Freire Barros
Coordinating Researcher
Helena Oliveira
Post-Doc Fellowship
João Pedro Fernandes Carvalho
Junior Researcher
Mara Guadalupe Freire Martins
Full professorGroups
G4 - Renewable Materials and Circular Economy
G5 - Biomimetic, Biological and Living Materials
Projects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
Engineering self-propelled cellulose-based Janus microrobots (Cell4Janus)
acknowledgements
This work was developed within the scope of the projects CICECO-Aveiro Institute of Materials, UIDB/50011/2020 (DOI 10.544 99/UIDB/50011/2020) , UIDP/50011/2020 (DOI 10.54499/UIDP/5 0011/2020) & LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020) , and CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020) financed by national funds through the FCT/MECTES (PIDDAC) . FCT is also acknowledged for research contracts under Scientific Employment Stimulus to C.S.R.F (DOI 10.54499/CEECIND/00464/2017/CP1459/CT0033) , C.V. (DOI 10.54499/2021.01571.CEECIND/CP1659/CT0024) and H.O. (DOI 10.54499/CEECIND/04050/2017/CP1459/CT0023) , and for the PhD scholarship of M.D.B. (2020.06571.BD, DOI 10.54499/2020.06571.BD) and J.P.F.C (2020.09018.BD, DOI 10.54499/2020.09018.BD) . The project Cell4Janus (PTDC/BII-BIO/1901/2021, DOI 10.54499/PTDC/BII-BIO/1901/2021) , financially supported by national funds (OE) through FCT/MCTES, is acknowledged for the research contract of W.M.F. The project be@t-Textile Bioeconomy (TC-C12-i01, Sustainable Bioeconomy No. 02/C12-i01.01/2022) , promoted by the Recovery and Resilience Plan (RRP) , Next Generation EU, for the period 2021-2026 is acknowledged for the research contract of M.C.T. The authors would also like to acknowledge Celbi-Celulose da Beira Industrial, SA and The Navigator Company for Funding the PhD scholarship of J. M. S. Confocal fluorescence image acquisition was performed in the LiM facility of iBiMED, a node of PPBI (Portuguese Platform of BioImaging) : POCI-01-0145-FEDER-022122.