abstract
Cellular Al2TiO5-Al2O3 composite ceramic monoliths were prepared and tested as a potential stationary phase for process chromatography. The material was characterized by measuring the pore size distribution and hydraulic permeability. The interstitial porosity, the axial dispersion coefficient, and the height equivalent to a theoretical plate were calculated by pulse tests under nonbinding conditions. The surface of the ceramic material was activated with graphene oxide and then functionalized with Cibacron Blue F3GA. The dynamic binding capacity of the functionalized columns was measured in chromatographic cycles by using bovine serum albumin (BSA) as the target molecule. The experiments showed that the proposed monoliths have a well-defined porous structure, leading to particularly interesting flow properties for chromatographic bioseparations, such as very high permeability and convection, as the main mass transport phenomenon. These results are encouraging for a possible future optimization of the functionalization procedure toward the development of efficient convective media for protein purification.
keywords
CIBACRON-BLUE F3GA; EMULSIFIED SUSPENSIONS; AFFINITY MEMBRANE; STATIONARY-PHASE; CHROMATOGRAPHY; PERFORMANCE; ADSORPTION; SEPARATION; DIFFUSION; TRANSPORT
subject category
Engineering
authors
Lalli, E; Onesti, R; Kovalevsky, AV; Portugal, CAM; Crespo, JG; Sarti, GC; Boi, C
our authors
Andrei Kavaleuski
Principal ResearcherProjects
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
acknowledgements
The authors acknowledge the Italian Ministry of University and Research. Carla A. M. Portugal and Joao G. Crespo acknowledge the help of FCT-MCTES through the Associated Laboratory for Sustainable Chemistry-Clean Processes and Technologies (LAQV) LA/P/0008/2020 (DOI 10.54499/LA/P/0008/2020), UIDP/50006/2020 (DOI 10.54499/UIDP/50006/2020), and UIDB/50006/2020 (DOI 10.54499/UIDB/50006/2020), financed by National funds from OE. Andrei V. Kovalevsky acknowledges the support of the project CICECO Aveiro Institute of Materials, UIDB/50011/2020 (DOI 10.54499/UIDB/50011/2020), UIDP/50011/2020 (DOI 10.54499/UIDP/50011/2020), and LA/P/0006/2020 (DOI 10.54499/LA/P/0006/2020), financed by National funds through the FCT/MCTES (PIDDAC) (ref UID/CTM/50011/2019), financed by the COMPETE 2020 Program and National Funds through the FCT/MEC and when applicable cofinanced by FEDER under the PT2020 Partnership Agreement. Eleonora Lalli acknowledges the support of the University of Bologna Marco Polo fellowship. The authors are also thankful to Nuno Costa (Universidade Nova de Lisboa) for his assistance with MIP analysis and to Manuela Melucci of ISOF, CNR of Bologna, for providing the graphene oxide powder.