Cloned Pseudomonas aeruginosa lipoxygenase as efficient approach for the clean conversion of linoleic acid into valuable hydroperoxides
authors Villaverde, JJ; Santos, SAO; Haarmann, T; Neto, CP; Simoes, MMQ; Domingues, MRM; Silvestre, AJD
nationality International
journal CHEMICAL ENGINEERING JOURNAL
author keywords Lipoxygenase; Linoleic acid; Oxidation; Fatty acid hydroperoxides; Modelling
keywords UNSATURATED FATTY-ACIDS; MISCANTHUS-X-GIGANTEUS; MANGANESE LIPOXYGENASE; SOYBEAN LIPOXYGENASE; EUCALYPTUS-GLOBULUS; TRANSFORMATION; OPTIMIZATION; OILS
abstract Linoleic acid (LA) was converted into hydroperoxides by a cloned lipoxygenase from bacterium Pseudomonas aeruginosa (PALOX) expressed in Trichoderma reesei. Its conversion was first analyzed for different LA concentrations. Then, a face-centred experimental design was used to study the effects of different variables (pH: 4.0-10.0; temperature: 5.0-45.0 degrees C; time: 2-34 h) on the conversion into hydroperoxides (13-hydroperoxy-(9Z,11E)-octadecadienoic, 9 -hydroperoxy-(10E,12Z)-octadecadienoic, 13 -hydroperoxy-(9E,11E)-octadecadienoic and 9-hydroperoxy-(10E,12E)-octadecadienoic acids) as well as on the residual LA. Fitting equations described satisfactorily the system behaviour and showed that pH and time were the most influencing variables. Finally, hydroperoxide yields above 75%, with high selectivity for the 13-hydroperoxy-(9Z,11E)-octadecadienoic acid, were obtained and results were compared with those previously obtained with Gaeumannomyces graminis tritici lipoxygenase (GGLOX). PALOX showed a maximum hydroperoxides formation at a dose 10 times lower than GGLOX. The PALOX system was more efficient than the GGLOX system although at the expense of longer reaction times and lower selectivity regarding the 13-hydroperoxy-(9Z,11E)-octadecadienoic acid. (C) 2013 Elsevier B.V. All rights reserved.
publisher ELSEVIER SCIENCE SA
issn 1385-8947
year published 2013
volume 231
beginning page 519
ending page 525
digital object identifier (doi) 10.1016/j.cej.2013.07.064
web of science category Engineering, Environmental; Engineering, Chemical
subject category Engineering
unique article identifier WOS:000326767900060
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