Antioxidant and antimicrobial films based on brewers spent grain arabinoxylans, nanocellulose and feruloylated compounds for active packaging


In this study, brewers spent grain (BSG) arabinoxylans-based nanocomposite films were prepared by solvent casting of arabinoxylans (AX) suspensions containing different amounts of nanofibrillated cellulose (NFC, 5, 10, 25, 50 and 75% mass fraction). The obtained nanocomposite films were homogeneous and presented thermal stability up to 230 degrees C and good mechanical properties (Young's modulus up to 7.5 GPa). Additionally, the films with 50% NFC were loaded with ferulic acid or feruloylated arabinoxylo-oligosaccharides enriched fraction from BSG (75 mg per g of film). This combination enhanced the UV-Vis barrier properties and imparted additional functionalities to the films, namely (i) antioxidant activity up to 90% (DPPH scavenging activity), (ii) antibacterial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, and (iii) antifungal activity towards the polymorphic fungus Candida albicans. Therefore, these fully biobased nanocomposite films show potential for application as active food packaging systems.




Chemistry; Food Science & Technology


Moreirinha, C; Vilela, C; Silva, NHCS; Pinto, RJB; Almeida, A; Rocha, MAM; Coelho, E; Coimbra, MA; Silvestre, AJD; Freire, CSR

nossos autores


This work was developed within the scope of the projects CICECO Aveiro Institute of Materials (UIDB/50011/2020 & UIDP/50011/2020), CESAM (UID/AMB/50017/2019), QOPNA (UID/QUI/00062/2019) and LAQV-REQUIMTE (UIDB/50006/2020), financed by national funds through the Portuguese Foundation for Science and Technology/MCTES, and when appropriate, co-financed by FEDER under the PT2020 Partnership Agreement. The research projects AgroForWealth (CENTRO-01-0145-FEDER-000001) and NANOBIOINKS (CENTRO-01-0145FEDER-031289) are acknowledged for the post-doctoral grant to C.M. and the research contract of N.H.C.S.S., respectively, both funded by Centro2020, through FEDER and PT2020. The research contracts of R.J. B.P. and E.C. are funded by national funds (OE), through FCT Fundacao para a Ciencia e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. FCT is also acknowledge for the research contracts under Scientific Employment Stimulus to C.S.R.F. (CEECIND/00464/2017) and C.V. (CEECIND/00263/2018).

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