Metabolic Effects of a Eucalyptus Bark Lipophilic Extract on Triple Negative Breast Cancer and Nontumor Breast Epithelial Cells
authors Guerra, AR; Soares, BIG; Freire, CSR; Silvestre, AJD; Duarte, MF; Duarte, IF
nationality International
journal JOURNAL OF PROTEOME RESEARCH
author keywords Eucalyptus (E. nitens) bark extracts; triple negative breast cancer (TNBC); cell metabolism; NMR metabolomics; glycolytic-oxidative balance
keywords OUTER BARKS; MITOCHONDRIAL; TARGET; GROWTH; ER
abstract In this work, untargeted metabolomics was used to unveil the impact of a Eucalyptus (Eucalyptus nitens) outer bark lipophilic extract on the metabolism of triple negative breast cancer (TNBC) and nontumor breast cells. Integrative analysis of culture medium, intracellular polar metabolites, and cellular lipids provided a comprehensive picture of cell metabolic adaptations, which enabled several hypotheses about the metabolic targets and pathways affected to be proposed. One of the most marked effects in MDA-MB-231 breast cancer cells, upon 48 h incubation with the E. nitens extract (15 mu g/mL), was the enhancement of the NAD(+)/NADH ratio, likely reflecting a shift to mitochondrial respiration, which appeared to be fueled by amino acids and fatty acids resulting from hydrolysis of neutral lipids (triglycerides and cholesteryl esters). Contrastingly, in MCF-10A breast epithelial cells, the E. nitens extract appeared to intensify glycolysis and the tricarboxylic acid cycle (resulting in a decreased NAD(+)/NADH ratio), while having no effect on the cell lipid composition. This knowledge improves the current understanding of the biological activity of E. nitens bark extracts and is potentially useful to promote their development in the field of TNBC anticancer therapy.
publisher AMER CHEMICAL SOC
issn 1535-3893
isbn 1535-3907
year published 2021
volume 20
issue 1
beginning page 565
ending page 575
digital object identifier (doi) 10.1021/acs.jproteome.0c00559
web of science category 11
subject category Biochemical Research Methods
unique article identifier WOS:000605145400050
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