FTIR Spectroscopy as a Tool to Study Age-Related Changes in Cardiac and Skeletal Muscle of Female C57BL/6J Mice
authors Magalhaes, S; Almeida, I; Martins, F; Camoes, F; Soares, AR; Goodfellow, BJ; Rebelo, S; Nunes, A
nationality International
author keywords aging fingerprint; aging muscle; spectroscopic profile
abstract Studying aging is important to further understand the molecular mechanisms underlying this physiological process and, ideally, to identify a panel of aging biomarkers. Animals, in particular mice, are often used in aging studies, since they mimic important features of human aging, age quickly, and are easy to manipulate. The present work describes the use of Fourier Transform Infrared (FTIR) spectroscopy to identify an age-related spectroscopic profile of the cardiac and skeletal muscle tissues of C57BL/6J female mice. We acquired ATR-FTIR spectra of cardiac and skeletal muscle at four different ages: 6; 12; 17 and 24 months (10 samples at each age) and analyzed the data using multivariate statistical tools (PCA and PLS) and peak intensity analyses. The results suggest deep changes in protein secondary structure in 24-month-old mice compared to both tissues in 6-month-old mice. Oligomeric structures decreased with age in both tissues, while intermolecular beta-sheet structures increased with aging in cardiac muscle but not in skeletal muscle. Despite FTIR spectroscopy being unable to identify the proteins responsible for these conformational changes, this study gives insights into the potential of FTIR to monitor the aging process and identify an age-specific spectroscopic signature.
publisher MDPI
isbn 1420-3049
year published 2021
volume 26
issue 21
digital object identifier (doi) 10.3390/molecules26216410
web of science category 12
subject category Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
unique article identifier WOS:000720278500001
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