Impact of growth medium salinity on galactoxylan exopolysaccharides of Porphyridium purpureum
authors Ferreira, AS; Mendonca, I; Povoa, I; Carvalho, H; Correia, A; Vilanova, M; Silva, TH; Coimbra, MA; Nunes, C
nationality International
journal ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
author keywords P. purpureum; Microalga; Culture salinity; Sulfated polysaccharides; Sulfation pattern; Immunostimulatory activity
keywords MOLECULAR-WEIGHT POLYSACCHARIDES; EXTRACELLULAR POLYSACCHARIDE; EICOSAPENTAENOIC ACID; CRUENTUM; MICROALGAE; PHYCOERYTHRIN; CARBOHYDRATE; EXTRACTION; EFFICIENCY; HYDROLYSIS
abstract Porphyridium purpureum is a red saline microalga rich in protein and floridean starch with the ability to excrete high amounts of sulfated polysaccharides (sEPS) into the growth medium. This microalga has been used for feeding fish in aquaculture. P. purpureum is easily cultivated and could change its growth rate and composition in response to environmental variations. Thus, the effect of growth medium salinity (18, 32, and 50 g/L NaCl) on P. purpureum cell growth, biomass composition, and extracellular polysaccharides production yield and chemical structure were evaluated. A maximum growth of an estimated 5.7 x 10(6) cells/mL achieved at a salinity of 32 g/L NaCl, after 19 days of growth. Different from the almost unchanged biomass composition of algal cells, the yield and structure of sEPS were affected by the salinity of the growth media. The salinity of the growth media led to a slight change of the sulfation pattern of the glucuronoglucogalactoxylan, as the sEPS produced by P. purpureum growing at lower salinity tended to be more sulfated in the O-3 position of xylose and the O-6 position of glucose, whereas the sEPS at higher salinity tended to be more sulfated in the O-4 position of xylose and glucose. The sEPS produced at higher salinity also revealed higher linear 2-Gal, 3-Gal, and 4-Gal residues. In all samples, the most representative sugars residues were constituted by t-Xyl, t-Xyl-4S, 3-Xyl, 4-Xyl, t-Glc, 3-Glc-6S, t-Gal, and 2,3,4-Gal. The sEPS showed immunostimulatory effect on B lymphocytes in vitro and could be produced at large scale at an outdoor 800 L-flat panel photobioreactor with higher yield. These results pave the way for the potential use of P. purpureum sEPS to enhance humoral immune activity of fish in aquaculture.
publisher ELSEVIER
issn 2211-9264
year published 2021
volume 59
digital object identifier (doi) 10.1016/j.algal.2021.102439
web of science category 12
subject category Biotechnology & Applied Microbiology
unique article identifier WOS:000694768800014
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journal analysis (jcr 2019):
journal impact factor 4.008
5 year journal impact factor 4.555
category normalized journal impact factor percentile 75.962
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