NMR metabolic composition profiling of high pressure pasteurized milk preserved by hyperbaric storage at room temperature


1H NMR spectroscopy was used to study the effect of hyperbaric storage (HS) on the chemical composition of high pressure pasteurized milk (HPP milk). A subsequent multivariate analysis (MVA) was applied to distinguish samples stored under different conditions. Three different pressure levels (50/75/100 MPa), at naturally variable uncontrolled room temperature (approximate to 20 degrees C), and up to 40 days of storage were compared with controls at atmospheric pressure and room temperature (AP/RT) or under refrigeration (RF). Only AP/RT samples presented spoilage compounds as lactate, methylamine and trimethylamine derived from microbial deterioration of milk. However, RF spectral profiles were closer to initial milk samples than the HS profiles, mainly in the aliphatic region which corresponds to an increase in soluble proteins for the HS samples. Additionally, MVA revealed higher levels of citrate, carnitine, lactose, and orotate in initial and RF samples, while HS samples had higher levels of acetate, N-acetylglucosamine, choline and galactose. In conclusion, although HS and RF samples were separated by MVA, there was no presence of microbial spoilage compounds in the spectra, being HS at RT an ecofriendlier alternative to preserve foods than traditional RF.




Food Science & Technology


Lemos, AT; Goodfellow, BJ; Delgadillo, I; Saraiva, JA

nossos autores


Thanks are due to the University of Aveiro and FCT/MCTES for the financial support for LAQV-REQUIMTE (UIDB/50006/2020) through national founds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement and for supporting the PhD grant of Alvaro T. Lemos (SFRH/BD/129848/2017) . The NMR spec-trometers are part of the National NMR Network (PTNMR) and are partially supported by Infrastructure Project No 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL and FCT through PIDDAC) . This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020 & UIDP/50011/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES.

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