Starch-based films doped with porphyrinoid photosensitizers for active skin wound healing

resumo

Starch is a biodegradable and biocompatible carbohydrate that, when combined with bioactive molecules, can be processed as biomimetic platforms with enhanced performance, allowing its use as active wound dressing materials. Porphyrinoid photosensitizers can tune the physicochemical/functional profile of biomacromolecules, allowing their use in anti-infective strategies. In this work, the feasibility of using the cationic 5,10,15,20-tetrakis (1-methylpyridinium-4-yl)porphyrin tetraiodide (TMPyP) to enhance the physicochemical, mechanical, antimicrobial performance, and wound healing ability of casted starch-based films was studied. TMPyP conferred a reddish coloration to the films, maintaining their pristine transparency. It increased by 87 % the films hydrophobicity and, depending on the TMPyP used, conferred mobility to the starch polymeric chains. Starch/TMPyPbased films effectively photoinactivated Escherichia coli (>99.99 %) and favored the wound healing process, even in the absence of light. Therefore, the incorporation of TMPyP into starch-based formulations revealed to be a promising strategy to tune the films compaction degree while giving rise to water tolerant and photosensitive biomaterials that can act as multitarget antimicrobial medical dressings and glycocarriers of active compounds relevant for effective skin wound healing.

palavras-chave

ANTIMICROBIAL PHOTODYNAMIC THERAPY; IN-VITRO; FIBERS; ACID; INACTIVATION; RESISTANCE; VIABILITY; MEMBRANE; RECOVERY; CANCER

categoria

Chemistry; Polymer Science

autores

Lopes, P; Joaquinito, ASM; Ribeiro, A; Moura, NMM; Gomes, ATP; Guerreiro, SG; Faustino, MAF; Almeida, A; Ferreira, P; Coimbra, MA; Neves, MGPMS; Gonçalves, I

Grupos

G2 - Materiais Fotónicos, Eletrónicos e Magnéticos
G4 - Materiais Renováveis e Economia Circular

Projectos

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

Projeto de Investigação Exploratória: Paula Ferreira (IF_Paula Ferreira)

Desenvolvimento de um composto bioplástico biodegradável à base dos subprodutos de batata (POTATOPLASTIC)

agradecimentos

The authors thank to University of Aveiro and FCT/MCT for the financial support provided to CICECO (UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020) , LAQV-REQUIMTE (UIDB/50006/2020 and UIDP/50006/2020) , CESAM (UIDP/50017/2020, UIDB/50017/2020, LA/P/0094/2020) , CEB (UIDB/04469/2020) , LABBELS and to PORP2PS2021) and PREVINE (FCT-PTDC/ASP-PES/29576/2017) , through national funds (OE) and where applicable co-financed by the FEDER-Operational Thematic Program for Competitiveness and Internationalization COMPETE 2020, within the PT2020 Partnership Agreement. Thanks are also due to the Portuguese NMR and Mass Networks. FCT also funded ASMJ PhD grant (2021.06854.BD) , Investigator FCT program (PF, IF/00300/2015) , and the Scientific Employment Stimulus program (IG, CEECIND/00430/2017; AR, 2021.02803.CEECIND) . NMMM thanks FCT for funding through program DL 57/2016 (CDL-CTTRI-048-88-ARH/2018) . The authors also acknowledge to POTATO-PLASTIC project (POCI-01-0247-FEDER-017938) , financed by FEDER through POCI, to Isolago Industria de Plasticos, S. A., the project leader, and to A Saloinha, Lda.com pany for providing the starchrich potato washing slurries.