Microscopic and Molecular Identification of Cyclospora cayetanensis and Cystoisospora belli in HIV-Infected People in Tabriz, Northwest of Iran

resumo

Opportunistic pathogens such as Cryptosporidium, Cystoisospora belli, and Cyclospora cayetanensis cause various gastrointestinal and non-digestive disorders in people with HIV/AIDS. These symptoms are especially severe in HIV-infected people who have a CD4+ count of less than 200 cells/mL. This study aimed to determine the prevalence of C. belli and C. cayetanensis infections among people living with HIV in Tabriz, northwest of Iran. This descriptive study was performed on 137 people with HIV who had been referred to behavioral disease counseling centers in Tabriz. Then, after receiving written consent, fecal samples were collected and evaluated for the detection of parasitic infections using direct methods and modified acid fast staining, as well as polymerase chain reaction (PCR).From the 137 fecal samples collected (98 males and 39 females, between 20 and 40 years old), 1.5% were positive for C. cayetanensis and 2.9% were positive for C. belli. Due to the prevalence of C. cayetanensis and C. belli in people with HIV in Tabriz, essential measures, including personal hygiene training for infection control and prevention, seem necessary.

palavras-chave

PARASITIC INFECTIONS; PREVALENCE; SANITATION; DIARRHEA; HYGIENE; WATER

categoria

Infectious Diseases; Parasitology; Tropical Medicine

autores

Ramezanzadeh, S; Barzegar, G; Osquee, HO; Pirestani, M; Mahami-Oskouei, M; Hajizadeh, M; Hosseini, SA; Oliveira, SRM; Agholi, M; Pereira, MD; Ahmadpour, E

nossos autores

agradecimentos

This study was part of the M.Sc. thesis of S.R and financially supported by Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran (Grant number 67748). We would like to express our gratitude all the staff of behavioral disease counseling centers in Tabriz who helped us in collecting and preparing the samples. Project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 and LA/P/0006/2020, financed by national funds through the FCT/MEC.

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