Extraction of natural colorants using supramolecular solvents composed of Triton X-114 and ionic liquids

resumo

The interest for natural colorants from microbial sources has increased in the last few years. However, the extraction of these compounds from complex biomasses/matrices is still a challenge for industrial applications, mainly due to the requirements of biocompatibility, sustainability, and efficiency. With this aim, supramolecular solvents (SUPRAS) composed of nonionic polyethylene glycol tert-octylphenyl ether (TX-114) and various cationic surfactants (n-alkyl-3-methyl imidazolium bromide ([Cnmim]Br, n = 10, 12, 14, 16), and tributyltetradecylphosphonium chloride ([P4,4,4,14]Cl) ionic liquids (ILs) and cetyltrimethylammonium bromide (CTAB)) were here studied for the extraction of red polyketides colorants from the fermented broth of Talaromyces amestolkiae. Firstly, the influence of ILs on the SUPRAS phase behavior was determined by measuring the cloud point temperature (TCP) and coarse-grained molecular dynamic (CG-MD) simulations. The results of extraction showed that for all SUPRAS the red colorant preferentially partitioned into the surfactant-rich (bottom) phase (partition coefficients, K > 10) with the highest partition using [C14mim]Br as a co-surfactant (K = 14.69 +/- 0.15). The systems studied also allowed high recovery efficiency of all mixed surfactant-based SUPRAS (>70 % of red colorant recovered in a single extraction step) with selective for the separation of the red colorant from the yellow (1.52 +/- 0.04) and orange (1.62 +/- 0.08) counterparts present in the fermented broth. The novel SUPRAS have demonstrated remarkable potential in extracting red colorants from fermented broth, without requiring harsh operating conditions. As such, these platforms offer an effective means of concentrating and prepurifying the red colorants, and hold promise for application to other molecules with similar chemical properties.

palavras-chave

AQUEOUS BIPHASIC SYSTEMS; COARSE-GRAINED MODEL; AGGREGATION BEHAVIOR; 2-PHASE SYSTEMS; NONIONIC SURFACTANTS; SUBMERGED CULTURE; FERMENTED BROTH; CO-SURFACTANTS; CLOUD POINT; SEPARATION

categoria

Engineering

autores

Nakamura, CN; Veríssimo, NVP; Oliveira, F; Frizzo, CP; Pérez-Sánchez, G; Coutinho, JAP; Pereira, JFB; Santos-Ebinuma, VG

Grupos

G4 - Materiais Renováveis e Economia Circular
G6 - Materiais Virtuais e Inteligência Artificial

Projectos

Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)

CICECO - Aveiro Institute of Materials (UIDB/50011/2020)

CICECO - Aveiro Institute of Materials (UIDP/50011/2020)

Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)

agradecimentos

This work was supported by Sao Paulo Research Foundation (FAPESP) - Brazil [Grant no. FAPESP 2019/15493-9, 2021/06686-8, 2021/09175-4 and Rio Grande do Sul State Foundation for Research Support, Brazil (Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul - FAPERGS) - grant no. 19/2551-0002273-5. J.F.B. Pereira acknowledges financial support from FAPESP through the project 2014/16424-7. C.P. Frizzo thanks the CNPq - proc. no. 432201/2018-1 and the fellowships from CNPq (grant no. 306389/2018-5). Valeria C. Santos-Ebinuma thanks the National Council of Scientific and Technology Development (CNPq) for the fellowship grant no312463/2021-9. N. V. Verissimo acknowledges funding from PROPG/PROPE UNESP No 05/2022. The authors also acknowledge the support from the CNPq (National Council for Scientific and Technological Development, Brazil) and the CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil), finance code 001. This work was partly developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the FCT/MEC and when appropriate co-financed by FEDER under the PT2020 Partnership Agreement. . CIEPQPF is supported by the FCT through the projects UIDB/EQU/00102/2020 and UIDP/EQU/00102/2020. G. Perez-Sanchez acknowledges the national funds (OE), through FCT - Fundacao para a Ciencia e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29th, changed by Law 57/2017, of July 19th.