resumo
The pulp and paper industry is a strategic sector to be converted into a forest-based biorefinery, taking advantage of know-how, existing infrastructures and well-established logistic operations to deal with a daily high volume of forest residues. Bioethanol production from Eucalyptus globulus bark previously pretreated by kraft pulping was assessed following two configurations at the bioreactor scale: separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). This last configuration was optimized by using a presaccharification and a fed-batch feeding (FB PS-SSF), allowing for a reduction in the enzyme dosage from 25 to 10 FPU gCH- 1, which is very advantageous from an economic point of view. For 10 FPU gCH-1 enzyme dosage and 20 % (w/v) total solids loading, an ethanol concentration of around 70.6 g L-1 was accomplished, corresponding to overall conversion efficiency and productivity of about 78 % and 1.35 g L-1 h-1, respectively. Increasing solids loading from 20 to 26 % (w/v), using the same enzyme dosage, resulted in the highest ethanol concentration (84.6 g L-1) despite the too-long assay and the decline in productivity. Overall, FB PS-SSF improved bioethanol concentration and allowed decreased enzymatic usage over SHF, maintaining high ethanol concentration.
palavras-chave
BIOETHANOL PRODUCTION; EUCALYPTUS-GLOBULUS; SUGARCANE BAGASSE; FERMENTATION; HYDROLYSIS; HARDWOOD; STRAW; LIGNOCELLULOSE; PRETREATMENT; CONVERSION
categoria
Energy & Fuels; Engineering
autores
Amandio, MST; Rocha, JMS; Xavier, AMRB
nossos autores
Ana Maria Rebelo Barreto Xavier
Professor Auxiliar
Mariana Soraia Tomás Amândio
Estudante de doutoramentoProjectos
InPaCTus - Innovative Products and Technologies from Eucalyptus Project (InPacTus)
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
This work was carried out under the Project InPaCTus - Innovative Products and Technologies from Eucalyptus. Project N.degrees 21874 funded by Portugal 2020 through European Regional Development Fund (ERDF) in the frame of COMPETE 2020 n degrees 246/AXIS II/2017. This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). Mariana S. T. Amandio acknowledges the support by Project InPaCTus for her PhD grant, POCI-01-0247-FEDER-021874. Authors would also like to thank the CIEPQPF -Strategic Research Centre Project UIDB/00102/2020 funded by the Fundacao para a Ciencia e Tecnologia (FCT). The authors are thankful to RAIZ -Instituto de Investigacao da Floresta e do Papel for supplying the pretreated Eucalyptus globulus bark and supporting the PT Provisional Patent Application No. PT118408.