resumo
This paper describes a unique, extremely sensitive biophotonic sensor with a three-tier S-tapered (3S) structure. It is designed for the real-time detection of Shigella flexneri (S. flexneri), a common foodborne pathogen that causes severe gastrointestinal diseases. The sensor development includes three distinct diameters of S-tapered structures. The performance of tapered sections was improved by using molybdenum disulfide nanosheets (MoS2-NSs), zinc oxide nanowires (ZnO-NWs), and photoluminescent bimetallic gold-copper nanoclusters (AuCu-NCs). These nanoparticles greatly improve the sensor's performance. The sensor is further functionalized using anti-S. flexneri antibodies, allowing for the precise detection and capture of the target bacterium. The results show that the sensor can detect S. flexneri rapidly and accurately, with a linear detection range of 1-10(8) colony-forming units per milliliter (CFU/ml) and a low detection limit of 4.412 CFU/ml. In addition, the sensor's ability to identify S. flexneri biofilms is demonstrated. Biofilm detection allows us to better understand and control biofilm concerns in the environment, equipment, and biomedical devices. Aptamer examines confirm the sensor's ability to detect S. flexneri from the lateral direction. This study makes a significant contribution to the field of biosensing because no biophotonic sensor has previously been developed specifically for the detection of S. flexneri, fulfilling a critical gap in the arena of food safety and pathogen detection. The 3S sensor's performance, robustness, and potential for practical applications make it an important addition to the field of photonics.
palavras-chave
SURFACE-PLASMON RESONANCE; FIBER SENSORS; NANOPARTICLES; NANOCOMPOSITE; IMMUNOSENSOR; BIOSENSORS; APTAMER; POLYMER; PROBE; ASSAY
categoria
Optics; Physics
autores
Xiao, LC; Singh, R; Nedoma, J; Wang, QL; Liu, FZ; Tosi, D; Marques, C; Zhang, BY; Kumar, S
nossos autores
Carlos Marques
Professor AuxiliarProjectos
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
agradecimentos
This work was supported by the Double-Hundred Talent Plan of Shandong Province, China; Special Construction Project Fund for Shandong Province Taishan Mountain Scholars; Liaocheng University (Grant No. 318052341); the Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China (Grant No. 2022KJ107); and the Science and Technology Plan of Youth Innovation Team for Universities of Shandong Province (Grant No. 2019KJJ019). This work was also developed within the scope of the projects CICECO (Grant Nos. LA/P/0006/2020, UIDB/50011/2020, and UIDP/50011/2020) and DigiAqua (Grant No. PTDC/EEI-EEE/0415/2021), financed by national funds through the [Portuguese Science and Technology Foundation/MCTES (FCT I.P.)]. The research was co-funded by financial support from the European Union under the REFRESH - Research Excellence for Region Sustainability and High-tech Industries Project No. CZ.10.03.01/00/22_003/0000048 via the Operational Programme Just Transition. This work was also supported by the Ministry of Education, Youth, and Sports of the Czech Republic conducted by the VSB-Technical University of Ostrava, under Grant Nos. SP2024/081 and SP2024/059.