resumo
Control over temperature in three space dimensions and time is of utmost importance in many contexts, including photothermal therapies, where it is expected to provide valuable insights and enhance clinical outcomes. Current techniques for noncontact temperature mapping during hyperthermia are restricted to 2D imaging, due to the lack of strategies able to provide images in suitable time frames for the inevitable large amount of information encompassed in 3D tomography. Iron selenide nanoparticles (NPs) are developed with high temperature sensitivity that, in combination with low-field magnetic resonance imaging, enable 3D temperature imaging in the approximate to 20 s time frame. This approach is suitable for monitoring and evaluating photothermal therapy applied to cellular assemblies, which typically occurs over a time frame of tens of minutes. In addition, iron selenide NPs behave, simultaneously, as photothermal and thermometric agents embedded in cellular models at concentrations where their cytotoxicity is low. This dual functionality enables to control the depth achieved by the therapy with a temperature accuracy <= 1 degrees C, enabling the detection and control of hot spots that would be otherwise overlooked. Overall, this is a universal approach for temperature mapping in 3D and for localized heating in virtually any living tissue.
palavras-chave
MRI; PARTICLES; CONTRAST
categoria
Chemistry; Science & Technology - Other Topics; Materials Science
autores
Soeiro, JF; Pereira, RA; Oliveira-Silva, R; Sousa, FL; Gaspar, VM; Mano, JF; Millán, A; Innocenti, C; Mariani, M; Lascialfari, A; Silva, NJO
nossos autores
Filipa Lourosa Sousa
Investigador Júnior
João Mano
Professor Catedrático
Nuno João Silva
Investigador Principal
Rui Pedro Oliveira Silva
Bolseiro de pós-Doutoramento
Rute A. Pereira
Estudante de doutoramento
Vítor Gaspar
Professor AuxiliarGrupos
G1 - Materiais Porosos e Nanossistemas
G2 - Materiais Fotónicos, Eletrónicos e Magnéticos
G5 - Materiais Biomiméticos, Biológicos e Vivos
Projectos
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
Development of 4D wireless thermometry to target tumor ablation (PTDC/NAN-MAT/3901/2020)
agradecimentos
This work was developed within the scope of the project CICECO-Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020, and LA/P/0006/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MEC (PIDDAC). The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the doctoral grants (2022.10039.BD, J.F.S., SFRH/BD/143320/2019, R.A.P.) and through an assistant researcher contract (DOI: 10.54499/2022.02106.CEE- CIND/CP1720/CT0028, V.M.G.). Additionally, this work was supported by grants PTDC/NAN-MAT/3901/2020 (DOI: 10.54499/PTDC/NAN-MAT/3901/2020 supported by POCI, FEDER, and FCT/MCTES) and grant ERC-2019-CoG-865437 from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program. The funding of project O2Cells-"Hybrid living bioengineered hierarchical constructs with self-oxygenating capability", (ref. 2022.04237.PTDC) is also acknowledged. M.M. and A.L. acknowledge the partial funding by the Istituto Nazionale di Fisica Nucleare (INFN) (project "Mather3D").