resumo
Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals that are recognized for their environmental persistence and potential toxicity. As regulatory pressure increases on legacy PFAS, emerging alternatives are being increasingly used. However, their environmental toxicological profiles remain poorly understood. The present study compares the biochemical effects of legacy (PFOS, PFOA, and PFNA) and emerging (GenX, PFBA, PFBS, and PFHxS) PFAS on the mussel species Mytilus galloprovincialis, using in vitro exposures of digestive gland (DG) and gills (G) tissues. The results highlighted tissue-specific responses, with greater biochemical variability observed in the DG. This was particularly evident under exposure to emerging PFAS, which appeared to induce oxidative stress disturbances and detoxification pathways, as indicated by increased acetylcholinesterase (AChE) and glutathione S-transferases activities and reduced total antioxidant capacity (TAC) levels. In contrast, the G showed a strong inhibition of both AChE and carboxylesterases activities and decreased TAC levels, possibly due to neurotoxic effects and compromised redox homeostasis. These findings suggest that emerging PFAS induce diverse and pronounced effects in the DG, whereas legacy PFAS generally cause less divergent biochemical responses. Further research into intra-group variability, especially among emerging PFAS, is essential for understanding their ecological risks and developing targeted regulatory frameworks.
palavras-chave
PERFLUOROALKYL SUBSTANCES PFASS; POLYCHAETE DIOPATRA-NEAPOLITANA; INTEGRATED BIOMARKER RESPONSE; POLYFLUOROALKYL SUBSTANCES; MYTILUS-GALLOPROVINCIALIS; CARBOXYLESTERASE ACTIVITY; WASTE-WATER; ACIDS PFAAS; CONTAMINANTS; EXPOSURE
categoria
Environmental Sciences & Ecology; Marine & Freshwater Biology
autores
Cunha, M; Bortot, C; Santovito, G; Nardi, A; Soares, AMVM; Gil, AM; Freitas, R
nossos autores
Ana Maria Pissarra Coelho Gil
Professor Associado com Agregação
Marta Barros Cunha
Estudante de doutoramentoProjectos
Collaboratory for Emerging Technologies, CoLab (EMERGING TECHNOLOGIES)
CICECO - Aveiro Institute of Materials (UIDB/50011/2020)
CICECO - Aveiro Institute of Materials (UIDP/50011/2020)
Associated Laboratory CICECO-Aveiro Institute of Materials (LA/P/0006/2020)
agradecimentos
Marta Cunha benefited from a PhD grant (2024.01100.BD) funded by National Funds through the Portuguese Science Foundation (Fundacao para a Ciencia e a Tecnologia, FCT). This work is funded by national funds through FCT - Fundacao para a Ciencia e a Tecnologia I. P., under the project/grant UID/50006 + LA/P/0094/2020 (doi: 10.5 4499/LA/P/0094/2020). This work was also developed within the scope of the project CICECO-Aveiro Institute of Materials UIDB/50011/2020 (doi: 10.54499/UIDB/50011/2020), UIDB/50011/2020, UIDP/50011/2020 (doi: 10.54499/UIDP/50011/2020) & LA/P/0006/2020, financed by national funds through the FCT/MCTES (PIDDAC) .